应用流式细胞术测定淡水、海洋沉积物中异养细菌前处理条件.PDFVIP

2008 3 Vol.3, 2008 3 , 307−312 Asian Journal of Ecotoxicology No.3, 307−312 应用流式细胞术测定淡水、海洋沉积物中异养细菌前处 理条件的选择 季倩，张经* 华东师范大学 河口海岸学国家重点实验室，上海 200062 摘要(FCM) FCM . FCM . FCM . 1mmol·L− 1 10min20w40KHz 1min30s 1 2800r·min− 1 3 . 关键词 文章编号1673-5897(2008)3-307-06 中图分类号Q93X172 文献标识码A Optimization in Pretreatment Conditions for Determination of Heterotrophic Bacteria from Freshwater and Marine Sediments Using Flow Cytometry * JI Qian, ZHANG Jing State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062 Received 12 March 2008 accepted 6 May 2008 Abstract: The Flow Cytometry (FCM) has been widely used to determine the heterotrophic bacteria in water environment presently. Also, it can be used to determine the heterotrophic bacteria in benthos environment after the appropriate pretreatment. The treatment of extracting sediment-attached bacteria to water environment is the key point of applying the FCM to the study of benthos. This paper discussed the methods of pretreatment for the FCM to detect the heterotrophic bacteria both in freshwater and marine sediments. Results showed that the relatively preferable pretreatment conditions for sediments were: sodium pyrophosphate as the dispersant with the final concentration of 1mmol·L− 1 and incubated 10min in dark; sonicate 1min in water bath at 20w, 40KHz power with a manual shake every 30s; and centrifugal extraction 3 times with 2800 r·min− 1 at room temperature. Keywords: flow cytometry; heterotrophic bacteria; sediment; pretreatment 收稿日期: 2008-03-12 录用日期: 2008