有机和常规红提葡萄附生菌的比较分析.PDFVIP

12 6 2011, Vol. 32, No. 03 食品科学 ※生物工程 有机和常规红提葡萄附生菌的比较分析 刘万振，武婷婷，生吉萍，申 琳* (中国农业大学食品科学与营养工程学院，北京 100083) 摘 要：以伊犁金山葡萄庄园的红提葡萄为原料，通过比较常规葡萄和有机葡萄不同器官表面附生菌群的差异，旨 在揭示常规和有机两种不同栽培模式对葡萄附生菌群的影响。葡萄附生菌的分离、筛选方法为平板培养法，微生 5 2 物的计数方法为最大或然数法。结果显示：葡萄根部附生菌的群落总数最大( 10 CFU/mm ) ，茎次之，果实最小 2 2 (约10 CFU/mm ) 。在果实上，有机葡萄附生真菌种类多于常规葡萄，而附生细菌种类与之相反。除葡萄叶没有 显著差异外，有机葡萄各器官( 根、茎、果实) 附生菌菌落总数均大于常规葡萄。初步表明，有机栽培可以更好 地保持生态环境中微生物群落的生物多样性。 关键词：红提葡萄；有机食品；附生菌；食品安全 Comparative Analysis of Epiphyte between Organic and Common Red Globe Grapes LIU Wan-zhen ，WU Ting-ting ，SHENG Ji-ping ，SHEN Lin * (College of Food Science and Nutritional Engineering, China Agricultural University, Beij ing 100083, China) Abstract：Both organic and conventional red globe grape were sampled from Jinshan vineyard in Yili. The effect of different farming models of conventional and organic farming models on the epiphyte distribution w as studied preliminarily in current p aper by comparin g the epiphytic b iodiversity in grape organ s. The agar-plate culture method was u sed for the microbial isolation , and the most-probab le-number (MPN ) was u sed for the enum eration of epiphyte population . The 5 2 result was as follows: the largest number of epiphytes located on the root (up to 10 CFU/mm ), and the stem took second 2 2 place, and the smallest on fruit (about 10 CFU/mm ). Besides, the amount of epiphytic fungi species of the organic grape was slightly h igh er th an th at of conv entional grap e; on