X线对胶质瘤细胞生长和放射敏感性相关基因表达影响及MGR2细胞株放射抗拒性诱导.pdfVIP

◇中山大学 x线对胶质瘤细胞株的影响及胶质瘤细胞MGR2放射抗拒性的诱导 11 After sublinewasnamed radiation months，the MGR2R(MGR2induction)． timesand curvesof andtheirsurvivalcells Doubling growth MGR2，T98G irradiationweredeterminedMTT curves， following by assay．Dose—survival andthesurvivalfractionat2 radiobiologicalparameters Gy(SF2)were determined and model．The byusingcolony-formingassay line-quadratic variationoftheircell was cell flow and cyclesinvestigatedbyusing cytometry ofserum-starvation．Severalassociated cyclesynchronization geneexpression with weredeterminedwesternblot inthesecells． radiosensitivity by analysis of determined Activity DNA--dependentproteinkinase(DNA·-PK)wasby DNA—PK of assay systemSigmaTechnology． Results： Therewasno of andthesurvivalcells morphologicalchangeMGR2，T98G irradiationunder timeof following byX-ray light MGR2 microscope．Doubling was3．6days，whileM-2(MGR22 followingGy 2 2 irradiation 5 followingGy times)，M-5(MGR2followingGyirradiation)were 4．3 timesofT98Gand days，4．8 T-3，T-3·3， days，4．8daysrespectively．D