绒山羊绒毛生长相关基因筛选、鉴定和多态性地分析.pdf

＜0.05); P9 primer existed in G-A mutation at NM_001206907:g.1015bp, which led to GGC-AGC of the 339th codon from Gly into Ser. The analysis indicated that interaction effect of the SNP and age was the marginal significant correlation(P=0.056) with cashmere fineness. (3) There were SSCP at P 14 primer and P 16 primer of TG gene. P14 primer of the exon 9 existed in C-T mutation at NM_173883:g.1895bp, which resulted in GCG-GTG of the 632th codon from Ala into Val. P16 primer of exon 11 existed in G-A mutation at NM_173883:g. 2849bp, which led to CGC-CAC of the 950th codon from Arg into His, which both were no significant effect on weight, kidding number and production cashmere traits (p ﹥0.05). P8 and P9 primes were not significance(P ＞0.05) with kidding number and birth weight. 2 、Selection, identification and characteristic analysis of skin differentially expressed gene on cashmere Goat (1) To select correlation genes which promoted cashmere growth embedding melatonin(MLT) in the telogen period by gene chip technology.To have selected 95 of differentially expressed genes, 61 of which were up-regulated expression, while 34 genes were down-regulated expression.Those abnormal expression genes were assorted by function, 18.33%, 47.78%, 33.89% of genes involved in cellular component, molecular function, biological process, respectively. (2) Analyses of signaling pathway showed that differentially expressed genes totally involved in 55 pieces of signaling pathway. CTNNB1 gene participated in WNT signaling pathway, and CYP2B gene related to metabolism of retinol and xenobiotics by cytochrome P450, which probably promoted cashmere growth and follicle development related with embedding MLT in the telo