现代分子生物学技术在动物肠道微生物多样性研究中应用.pdfVIP

应用与环境生物学报 2005, 11( 3): 381~ 387 Ch in J App l E n vir on B io l= I SSN 2005206225 * * * 冯 霞 殷幼平 王中康 (重庆大学生物工程学院教育部生物力学与组织工程重点实验室 重庆 400044) 现代分子生物技术 蓬勃发展解决了不可培养微生物研究 难题, 使得肠道微生物 研究进入一个新 阶 段.本文主要介绍了基于 16S rRNA 分子分析技术,包括 DGGE (变性梯度凝胶电泳 ), TGGE (温度梯度凝胶电泳), SSCP (单链构象多态性), RFLP (限制性片段长度多态性 )等肠道微生物研究工作中常用 分子生物学技术方法、适 用范围及可能 发展方向,为动物肠道微生物区系 进一步研究及开发应用提供帮助. 表 1参 42 16S rRNA; 16S223S rDNA间区; 遗传指纹图谱技术; 肠道微生物多样性 CLC 48: 81 APPLICATION OFMOLECULAR BIOLOGICAL TECHNIQUES IN * RESEARCH OFM ICROBIAL DIVERSITY IN ANIMAL INTESTINES * * FENG Xia, YIN Youping WANG Zhongkang (KeyLab for Biomechan ics Tissue Engin eering of theMinistry of Education, Bio2engineering College, Ch ongqing University, Chongqing 400044, China) Abstract Molecularbiological techniqueswere applied inm icro2ecological research and resolved lots ofdifficulties in studying them icroorganisms, which could notbe cultured. So the research of intestinem icrobes gained anew phase. This paper pres2 ents am ini2review on the molecular ecological techniques used for m icrobial diversity research in anmi al guts based on 16S rRNA, aswell as 16S223S rDNA Intergenic Spacer Region, RAPD, LMW RNA, fluorescence in situ hybridization, including a series ofgenetic fingerpr