南瓜花粉母细胞减数分裂及雄配子体发育.pdfVIP

Agricultural Science＆Technology，2012，13(3)：547-550 Copyright )2012，Information Institute of HAAS．All rights reserved Agronomy and Forestry Pol len Mother Cel l M iosis and Male Gametophyte Development of Pumpkin Qunxiang CUI ，Weidong LIU，Qian WANG，Muzhu XIAO Jinling Institute of Technology，Nanjing 21 0038，China buds were fixed for 24 h by using Abstract『Obiective1 Pollen mother cel J miosis and male gametophyte development of modified Kano fixative[Sj．soaked in 70％ pumpkin were obsewed in this study，to provide some cytological basis for pumpkin anther or microspore culture．fMethod1 Ehrlich’s hematoxylin staining—methyI salicylate ethanoI and stored at 4 oC In a refriger— clearing technique was used for observation and research of the variation of cell ator for use． structure and chromosomal behavior during pollen mother cell miosis and male ga— Experimental methods metophyte development of‘Tianhong’pumpkin．『Result1 The meiosis in pollen moth— Staining and clearing procedures er cells of pumpkin was simultaneous cytokinesis．In the process of nuclear division， nuclear membrane and nucleolus ofpumpkin pollen mother cells gradually disap- were in accordance with the method peared in the metaphase l and reappeared in telophase 1．phragmoplast formed proposed by Zhang et a1．[5】with slight between the two ~enerated crescent—shaped nuclejwithout cel1 wal1．the phragmo— modification．After staining．1 mol／L HC1 plast gradually disappeared jn the metaphase II and reappeared in telo