不同基材之微结构与软硬度对癌细胞移动的影响-2016临床工程研讨会.docVIP

不同基材之微結構與軟硬度對癌細胞移動的影響 The Influence of Substrata Microstructure and Rigidity on Cancer Cells Migration 陳玟綺Wen-Chi Chen1, 王國銘Kuo-Ming Wang1, 謝清河Patrick Chin-Ho Hsieh2, 李佳徽Chia-Hui Li1, 葉明龍Ming-Long Yeh1* 1國立成功大學醫學工程研究所 Institute of Biomedical Engineering, National Cheng Kung University, Taiwan 2國立成功大學臨床醫學研究所 Institute of Clinical Medicine, National Cheng Kung University, Taiwan * Corresponding Email: mlyeh@mail.ncku.edu.tw 中文摘要 細胞在進行這些行為時，會受到基材軟硬度或表面形態所影響。而細胞移動的速率對癌細胞的移轉是很重要的因素，故本研究欲探討使用軟硬程度不同的基材搭配上不同表面特定形態，對細胞移動能力之影響。 本研究使用人類黑色素腫瘤細胞(Human melanoma)，以10:1及20:1兩種比例所調配出的聚二甲基矽氧烷 (PDMS)為基底材料，使用微機電製程(MEMS)技術翻模出兩種不同的表面型態, 平頂圓錐及長橫溝，分別具有兩種不同線寬為1 μm及6 μm，與玻璃基材對照。細胞於基材穩固貼附12小時後，連續觀察細胞位置6小時。 實驗結果顯示，細胞均會沿著與長橫溝平行的方向移動，且隨著線寬的增加而增加，在平頂圓柱及平的表面上則會無規則的排列與移動。細胞隨長橫溝平行移動的比率，隨著線寬的減小而下降，且細胞貼附面積受平頂圓柱影響最為顯著。在所有基材上，細胞在玻璃上移動的最慢，在具有較軟的PDMS上移動的比硬的PDMS快，且細胞在較軟的PDMS所翻模出的6 μm平頂圓柱上移動的最快 (0.91 ± 0.06 μm/min)。 關鍵詞：細胞移動、微機電製程、基材表面型態、基材軟硬度 Abstract It is known several physical factors like stiffness and surface morphology of the substrata are also thought to influence cell behaviors. The purpose of this study is to measure the cell migration under different stiffness and structure of the substrata. Human melanoma cells were used for this study. The polydimethylsiloxane (PDMS) with ratios of silicon elastomer/curing agent concentration at 10:1 and 20:1 were fabricated for soft substrata. The surface patterns in the study included cones and grooves with line width at 1 μm and 6 μm. After cells were seeded on the substrates for 12 hours, the cell images were taken by optical microscopy every 5 minutes for 6 hours. Directional migration increased with larger line width, parallel migration to the groove and random orientation and migration to the cones and flat substrata. The frequency of cells moving along the groove decreased with smaller line width. Flat-top cone was the best surface morphology to influence cell area. The migration speed on glass was the lowest and significantly faster on soft PDMS when