化妆品中壬基酚的固相萃取-超高效液相色谱测定法.pdfVIP

环境与健康杂志2014年 7月第 3l卷第 7期 JEnvironHealth，July2014，Vo1．31，No．7 · 技术与方法 · 化妆品中壬基酚的固相萃取一超高效液相色谱测定法 王萍，李洁 北京市疾病预防控制中心环境卫生所，北京 100013 摘要：目的 建立化妆品中壬基酚的超高效液相色谱测定法。方法 样品经固相萃取后，用超高效液相色谱 (PDA检测 器)进行检测，色谱柱为WatersACQUITYUPLCMTBEH苯基柱(50mmx2．1mm，1．7Ixm)，定量检测波长为 224nm。结果 该方法的线性范围为2～50Ixg／ml，r=0．9995，检出限为 1Ixg／g，回收率为92％～102％，RSD9％。结论 该方法准确、可靠 ， 适合化妆品中壬基酚的日常检测。 关键词：壬基酚；化妆品；固相萃取；超高效液相色谱 中图分类号：0657．7 文献标志码：A 文章编号：1001—5914(2014)07—0610—03 Determinationofnonylphenolincosmeticsbyultra-highperformanceliquidchromatographywith solidphaseeXtraction WANG Ping，LIJie InstituteofEnvironmentalHealth，BeringCenterforDiseaseControlandPrevention，Bering100013，China Correspondingauthor：LI e。E—m Z：ljie100@J63．tom Abstract：0bjectiveToestablishanultra-performanceliquidchromatographywithsolidphaseextractionmethodforthe determinationofnonylphenolincosmetics．MethodsThesampleswereprocessedbysolidphaseextractionanddetemr inedby ultra—high perfomr ance liquid chromatography with PDA detector．The separation ofnonylphenolwascarried outby an ACQUITYUPLC BEHPhenylcolumn(50mmx2．1mm，1．7 m)andquantificationwasbasedondetectionwavelengthat224 mn．Results Thelinearrangesofthecalibrationcurvesofrnonylphenolwas2—50~g／m1．Thecorrelation coefficientsofthe standardcurveswas0．9995；，Illelimitsofdetection (LODs)ofrnonylphenolwas1~g／g；neaveragerecoverieswere92％一 102％ ．Relative standard deviationswere alllessthan 9％ ．Conclusion The method issimple．rapid and can meetthe requirementofroutineanalysisofthenonylphenolincosmetics． Keywords：Nonylphenol；Cosmetics；Solidphaseextraction；Ultra—perfomr anceliquidchromatography 壬基酚(NP)是一种多用途的非离子表面活性剂， 色谱工