BDNF、GRIN1基因与双相情感障碍的关联研究.docxVIP

HEREDITAS (Beijing) 2007 年 1 月, 29(1): 41―46研究报告ISSN 0253-9772/DOI: 10.1360/yc-007-0041BDNF、GRIN1 基因与双相情感障碍的关联研究刘敏 1, 凌四海 1, 李文标 1, 王传跃 1, 陈大方 2, 王刚 11. 首都医科大学附属北京安定医院科研室, 北京 100088;2. 北京大学公共卫生学院流行病学与卫生统计学系, 北京 100083摘要: 为了探讨 GRIN1 和 BDNF 基因的遗传变异在双相情感障碍疾病中的相关作用, 从 GRIN1、BDNF 基因上各取 2 个 SNP 位点, 采用 TaqMan 法对 100 例双相情感障碍患者和 100 例健康人进行了单核苷酸多态性分析, 比 较两组基因型频率的差异, 并使用软件 SHEsis 进行单体型分析。结果发现 GRIN1 基因上的 rs2301363 和 hcv1840191 与双相情感障碍发病的关联有统计学意义(P0.05), 它们形成的单倍型 T/G 在两组人群中的分布差 异亦有统计学意义(P0.05)。而 BDNF 基因上的 rs7103411 和 rs6265 与双相情感障碍发病无统计学意义。实验结 果表明 GRIN1 基因是双相情感障碍的易感基因之一。关键词: 双相情感障碍; N-甲基-D-天冬氨酸; 谷氨酸受体离子化 NMDA1 受体基因; 脑衍生神经营养因子; 单核苷酸多态性; 单体型分析An association study between grin1, bdnf genes and bipolar disorderLIU Min1, LING Si-Hai1, LI Wen-Biao1, WANG Chuan-Yue1, CHEN Da-Fang2, WANG Gang11. Beijing Anding Hospital Affiliated to the Capital University of Medical Sciences, Beijing 100088, China;2. Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing 100083, ChinaAbstract: To evaluate the role of inherited gene variations in GRIN1 (glutamate receptor, ionotropic NMDA1), BDNF(brain derived neurotrophic factor) genes in human bipolar disorder, we selected 4 single nucleotide polymorphisms in GRIN1, BDNF (2 SNPs in each gene) and made SNPs analysis in 100 unrelated cases and 100 controls by TaqMan. Then we compared genotypes differences between cases and controls. The software SHEsis was also used to make haplotype analysis. The significant results were obtained, showing that the SNPs in GRIN1 gene were related to the BP (P 0.05). In addition, the combined haplotype T/G had a significant difference in the two groups (P 0.05). The SNPs in BDNF geneshowed no statistical significance. These results confirm that the GRIN1 gene confers susceptibility to bipolar disorder.Keywords: bipolor disorder (BP); N-methyl-D-aspartate (NMDA); glutamate receptor, ionotropic NMDA1 gene (GRIN1);N-methyl-D-aspartate receptor, brain-deri