ATRFTIR和衰减全反射光谱的原理.pptVIP

Attenuated Total Reflection Flourier transformed Infrared Spectroscopy ATR-FTIR catalogue Brief introduction of ATR How ATR works applications conclusions Brief introduction of ATR Attenuated Total Reflectance (ATR) is an IR sampling technique. It measures the changes in an infrared beam with totally internal reflection when it comes into contact with a sample. ATR is used for surface analysis of solids and liquids. ATR generally allows qualitative or quantitative analysis of samples with little or no sample preparation which greatly speeds sample analysis. How ATR Works With ATR sampling we direct the IR beam into a crystal of relatively higher refractive index at a certain angle. The IR beam reflects from the internal surface of the crystal and creates an evanescent wave. It extends beyond the surface of the crystal into the sample held in contact with the crystal. where n2 is the refractive index of the sample, n1 is the refractive index of the crystal and θc is the critical angle. 1 when exceeding the critical angle, we will get a totally reflection of the IR beam. 2 n1n2 ,the crystal has a relatively high refractive index. Light path in?the?ATR setup The sample is put on top of the blue crystal. The IR beam picks up a signal as it passes a few micron into the sample and creates an evanescent wave. While the evanescent wave extends only 0.5-5 μm beyond the crystal surface and into the sample. schematic illustration Equipment to push the sample onto the crystal This can be achieved by pressing the sample down on the crystal. If the substance under study is a liquid, it is simply dropped onto the crystal, thereby?obtaining optimal contact. Therefore it is important to obtain good contact between the crystal and the sample. Tinfoil Traditional IR spectroscopy need preparation of the sample. schematic illustration First a couple of milligram of sample is mixed with about 200 mg of KBr powder. Then the mixture is pressed into a tab