人胎儿肠壁结缔组织对骨髓间充质干细胞分化的诱导-第三军医大学学报.DOCVIP

胎儿肠壁结缔组织对骨髓间充质干细胞分化的诱导王蓓张建华姜蓉汪维伟重庆医科大学组织胚胎学教研室临床学院妇产科重庆 400016 [摘要] 目的 观察胎儿肠壁结缔组织能否诱导骨髓间充质干细胞（BMSCs）分化上皮细胞。方法 肠块将DAPI标记的3代BMSCs种植于组织块面，EGF的A、B组；另设BMSCs细胞爬片的C、D对照组。体外培养至第12d观察荧光显微镜下组织块表面有DAPI荧光标记细胞，HE染色显示细胞呈上皮样形态免疫组化染色显示表面细胞表达CK及CK20组织块有EGF表达DAPI和红色CK或者绿色EMA双荧光标记阳性细胞PAS反应显示在细胞与结缔组织之间有基膜样肠壁结缔组织能诱导BMSCs分化为上皮细胞，内源性EGF可能是其诱导因素之一。 肠骨髓间充质干细胞分化 中图分类号：Q254INDUCTION OF FETAL INTESTINAL CONNECTIVE TISSUE ON THE DIFFERENTIATION OF BONE MARROW-DERIVED MESENCHYMAL STEM CELLS Duan Jiang-jie Wang Bei Zhang Jian-hua Jiang Rong Wang Wei-wei (Department of Histology and Embryology, Chongqing Medical University） [Abstract] Objective: To observe the possibility of that the bone marrow mesenchymal stem cells (BMSCs) induced by fetal intestinal connective tissue differentiate into epithelial cells. Methods: The fetal intestinal tissue lumps contained mucosa and submucosa were prepared, and removed the intestinal epithelium with enzymatic digestion. The 3rd generation cells of fetal BMSCs labeled with DAPI were planted on the surface of the bowel submucosa, 基金项目：重庆医科大学创新基金（CX200307） 作者简介：段江洁（1972-），女，重庆市人，汉族，硕士研究生, 主要从事干细胞分化研究 通讯作者：汪维伟，重庆医科大学组胚教研室教授，硕士生导师，400016 and divided as groups A and B added or not added EGF；the BMSCs attached on cover glass were set up as control groups of C and D. Then, cultured them in vitro for 12d，the morphological，histochymical and immunohistochemical analysis was observed with light microscope and CLSM. Results: ①The DAPI marked cells could be seen on the surface of tissue lumps by fluorescence microscope, and in the same section stained by HE, these cells have a morphous like epithelial cell. ②Immunohistochemical stain show that the cells on the surface of tissue lumpin groups A、B and group C expressed Ckand CK20，but negative in group D; There was the expression of EGF in intestinal connective tissue. The double fluorescent labelling cells with blue DAPI and red CK or green EMA could be seen on the surface of tissue lumps observed by CLSM. ③The bas