多肽配体技术在植物功能基因组学中的应用前景.pdfVIP

HEREDITAS (Beijing) 2010 6 , 32(6): 548―554 ISSN 0253-9772 综述 DOI: 10.3724/SP.J.1005.2010.00548 多肽配体技术在植物功能基因组学中的应用前景 公丕昌, 王丽, 贺超英 , , 100093 人工智能配体或适配体(Aptamer)技术是近年来兴起的一项特异性极强的基因干扰技术。通过人工合成 特异的智能配体结合靶基因的蛋白产物, 达到特异干扰靶基因的生物学功能, 这是人工智能配体技术的基本设 想。文章综述了多肽配体(Peptide aptamer)技术在基因功能验证中的主要进展, 着重阐明它在植物基因功能验证 和作物抗病毒育种中的应用前景, 并提出克服该技术主要风险对策。 多肽配体技术; 化学遗传学; 蛋白干扰; 功能基因组学 Peptide aptamer: a powerful potential tool in plant functional genomics GONG Pi-Chang, WANG Li, HE Chao-Ying State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany , Chinese Academy of Sciences, Beijing 100093, China Abstract: Aptamer is a newly invented tool for gene interference in vivo with highly specificity. An aptamer is designed to specifically target a protein and inhibit its biochemical roles for ascertaining its biological functions. Here, we briefly re- view the major progress of peptide aptamer in validating gene function, and emphasize its promising application in both plant functional genomics and peptide-mediated broad-spectrum plant resistance to diverse phytoviruses. The strategies to overcome the potential risks are covered as well. Keywords: peptide aptamer; chemical genetics; protein interference; functional genomics [1~3] (Reverse genetics) , RNAi (RNA interference) , , ; (Forward genetics) GW2