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Biochemical Engineering Journal 110 (2016) 115–124
Contents lists available at ScienceDirect
Biochemical Engineering Journal
j o u r n a l h o m e p a ge : w w w. elsev /locate/bej
Regular article
Methylene blue enhances the anaerobic decolorization and
detoxication of azo dye by Shewanella onediensis MR-1
Weijie Liu ∗,1, Liang Liu 1, Cong Liu, Yan Hao, Hao Yang, Bo Yuan, Jihong Jiang ∗∗
School of Life Science, The Key Laboratory of Biotechnology for Medicinal Plant ofJiangsu Province, Jiangsu Normal University, Xuzhou 221116, Jiangsu
Province, China
a r t i c l e i n f o a b s t r a c t
Article history: Azo dyes are recalcitrant pollutants that constitute a significant burden to the environment. This study
Received 30 September 2015 focused on decoloring azo dye Congo red using Shewanella oneidensis MR-1 and exploring methylene
Received in revised form 24 January 2016 blue as electron shuttle to enhance its decolorization and detoxication efficiency. The effects of physico-
Accepted 15 February 2016 chemical parameters on anaerobic decolorization were investigated. The results showed that the suitable
Available online 18 February 2016
pH, temperature and NaCl concentration range for decolorization were 6.0–8.5, 28–40 ◦ C and 0–20 g/L,
respectively. Under the optimal conditions, 99.25% decolorization of up to 2 g/L Congo red was achieved
Keywords:
within 24 h. Nitrite, nitrate and oxygen could significantly inh
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