细胞生物学-钟桂生2017-3--ZHONG Cytoskeleton-5-actin -case.pdfVIP

Cytoskeleton • 1. Basic introduction to intermediate filaments, microtubules and actin • 2. Cases for actin/microtubule structure in neurons Actin and accessory proteins Actin binding proteins (ABPs) 3 groups: banding and cross linking proteins regulatory proteins: Organisation of polymerization/depolymerization, actin filaments severing proteins,capping proteins  Motor proteins - sliding on MF (myosin) Sliding Neurons: 1. Actin waves 2. Actin ring Waves are associated with axonogenesis Waves transport actin. Cofilin activity may be involved in wave propagation. Inhibition of myosin II enhances axonogenesis, neurite branching and increases the prevalence of wave-like structures. Waves occur in Thy1-YFP labeled neurons in hippocampal slices. Neurons: 1. Actin waves 2. Actin ring Neuronal polarization Dendrites Axon Cell body Actin cytoskeleton structures in neurons Function of actin cytoskeleton in neurons • Establishment of neural polarity • Growth and stablization of axon and dendrites •Trafficking of cargoes to specific neurites • Plasticity of synapses Destabilization of actin cytoskeleton and neurodegenerative diseases • Parkinson Disease, Alzheimer’s disease •, and Amyotropic later sclerosis (ALS) EM imaging of actin in dendritic spine Korobova, et al, Mol. Bio. Cell, 2010 Imaging actin cytoskeleton with Super- resolution Fluorescent