蚊幼虫病原真菌贵阳腐霉的生物学研究.pdfVIP

26 3 380~388 2007 Mycosystema Biological studies on Pythium guiyangense, a fungal pathogen of mosquito larvae * HUANG Sheng-Wen SU Xiao-Qing (Department of Biology, Guiyang Medical College, Guiyang 550004, China) Abstract: Biological characteristics of Pythium guiyangense (Pythiaceae, Peronosporales, Oomycota), a species of mosquito-killing fungus, were studied. Hyphal growth rate, sporulation and virulence to the first instar larvae of Culex quinquefaciatus were used as assessing indexes. Seven kinds of artificial media with different carbon and nitrogen sources were used in the study. The effect of different pH values and temperature, and different light-dark alternation treatments on the fungal growth were investigated. The results showed that 5 ~35 was the suitable temperature range for the fungal growth, with optimal temperatures of 25 ~30 ; suitable pH values were 5~12, with optimal pH range of 9~11 for development of both hyphae and zoospores; the media ranked by favorite for fungus growth were Czapek’s, SFE, PYG, KPYG2, SDAY, CMA, PDA, while SFE was the best medium for fungal sporulation and increase of pathogenicity to mosquitoes; glucose, fructose, lactose, sucrose, maltose, mannose and soluble starch were suitable hydrocarbon sources; media containing organic nitrogen were much better than those containing inorganic nitrogen; light-dark alternation treatments did not show influence on the fungal growth, but ultraviolet illumination has obviously inhibitory effect. Key words: mosquito, biocontrol, Pythium guiyangense, biological characteristics 550004 Pythium guiyangense Culex quinquefaciatus1