论着·移植肾急性排斥反应相关免疫基因芯片的应用-实用器官移植.pdfVIP

实用器官移植电子杂志  2016年9月第4卷第5期  Pract J Organ Transplant（Electronic Version）， September 2016，Vol.4，No.5 ·291· ·论著· 移植肾急性排斥反应相关 免疫基因芯片的应用 宋文利，郑建明，莫春柏，冯刚 （天津市第一中心医院器官移植中心，天津 300192） 【摘要】 目的 通过基因芯片技术研究与急性排斥反应 （AR ）相关的差异表达基因，为提高肾移 植术后AR 的早期诊断水平及肾移植的疗效提供实验依据。方法 以手术当日和移植肾穿刺活检当日收 集的外周血作为对照血样和实验血样，提取总RNA ，反转录合成cDNA ，芯片杂交，筛选差异表达基因。 结果 发现了外周血淋巴细胞中与AR 可能相关的免疫基因，其中外周血淋巴细胞免疫相关基因差异表达 上调20个基因，下调 11个基因。结论 外周血淋巴细胞中存在与AR 可能相关的免疫基因，筛选出AR 时 表达量出现差异的基因。 【关键词】 肾移植 ； 急性排斥反应 ； 基因芯片 Application of immune gene array related to acute rejection of renal transplantation operation Song Wenli ， Zheng Jianming ， Mo Chunbai ，Feng Gang. Organ Transp lantation Center ，Tianj in First Center Hosp ital ，Tianj in 300192 ，China Corresp onding author ：Song Wenli ，Email ：songwenli@vip . 【Abstract 】 Objective To explore the deference expression of genes related with acute rejection （AR ） by gene array ，in order to provide experimental evidence for improve the level of early diagnosis and the efficacy of renal transplantation. Methods The peripheral blood were collected at the day of operation and kidney puncture biopsy and were served as the control sample and lab sample ， total RNA were extracted ，cDNA probe were synthesized by reverse transcription ，array hybridization were implemented ，differentially expressed genes were screened. Results The study discovered immune gene in peripheral blood lymph cell which was possibly related with AR ，there were 20 up-regulated and 11 down-regulated immune associated gene differential expression in peripheral blood lymph cell. Conclusion There were immune gene with AR may be related in perioheral blood lymph cell and the immune gene whose express-dose appears difference at the