十精丸血清药物化学研究.PDF

·914 · 国际中医中药杂志2017年10月第39卷第10期 Int J Trad Chin Med, October 2017, Vol. 39, No.10 ·实验与方药 · 十精丸血清药物化学研究 杨锐 邹德龙 陈丽娟 【摘要】 目的 利用中药血清药物化学的方法研究十精丸的入血成分。方法 建立口服十精丸后 大鼠血清的 HPLC 指纹图谱，比较十精丸、缺味方、单味药及给药后的血清样品，确定口服十精丸后大鼠 血中移行成分及来源生药。结果 十精丸给药后血中出现 14 个移行成分，4 个为血中固有成分，7 个为十 精丸中原型成分，3 个为代谢产物。结论 阐明了十精丸的部分入血移行成分，为进一步研究十精丸的药 效物质基础提供参考。 【关键词】 DNA 指纹法；十精丸；移行成分 Preliminary study on serum pharmacochemistry of Shijing pill Yang Rui, Zou Delong, Chen Lijuan. 2014 Graduate Students, Graduate College of Heilongjiang University of Chinese Medicine, Harbin 150040, China Corresponding author: Chen Lijuan, Jiamusi College of Heilongjiang University of Chinese Medicine, Jiamusi 154007, China; Email: chljxx@163.com 【Abstract 】 Objective To conduct serum pharmacochemistry study on Shijing pill. Methods HPLC fingerprints of serum in rats after taking Shijing pill were established,the serun samples after taking Shijing pill, decoction without one of the component drugs and single crude drug were compared,the transitional constituents absorbed into the blood and the original crude after taking Shijing pill was determined. Results Fourteen transitional constituents were detected in rat blood after taking Shijing pill,four of which were permanent component of blood,seven of which were prototype constituents,three of which were metabolites. Conclusions Some of chemical composition of Shijing pill was clarified preliminarily,that contributes to further studying pharmacodynamic meterial foundation of Shijing pill. 【Key words 】 DNA Fingerprinting; Shijing pill; Transitional constituents 十精丸出自明代《普济方•补虚驻颜色附论》， 中药血清药物化学是以中药口服给药后的血 是中医益寿延年、驻颜防衰之传统名方，由菟丝