羊睾丸提取液对铅损伤小鼠精子顶体形成的影响.pdfVIP

　 军 杂 2012年11月1 日　第37卷　第11期 870 解放 医学 志　 羊睾丸提取液对铅损伤小鼠精子顶体形成的影响 田洪艳，李质馨，王弘珺，朱辛为，潘晓燕，刘忠平，林冬静 [摘要]　目的　观察羊睾丸提取液对铅损伤小鼠精子顶体形成的影响。方法　成年健康清洁级ICR雄性小鼠30只， 随机分为对照组、模型组和给药组，每组10只。模型组采用醋酸铅(30mg/kg)灌胃建立睾丸损伤模型，给药组在醋酸铅 灌胃的同时腹腔注射羊睾丸提取液0.5ml ，对照组采用等体积蒸馏水灌胃及腹腔注射，每天1次，共21d 。经心脏行甲醛 灌流固定，制备睾丸标本，PAS染色，光镜下观察圆形精子细胞期顶体的形态学变化。结果　与对照组相比，模型组精 子顶体形成障碍，顶体囊泡不完整，膜皱缩，较粗糙，结构模糊；给药组精子顶体形成接近对照组，顶体囊泡形状较规 则，膜平滑。结论　羊睾丸提取液可保护小鼠精子顶体在形成过程中免受铅的损害，从而维持其正常的结构及功能。 [关键词]　睾丸提取物；铅中毒；精细胞；顶体 [中图分类号]　R321.1 [文献标志码]　A [文章编号]　0577-7402(2012)11-0870-04 Effect of caprine testicular extract on acrosome formation of spermatids of lead-injured mice * TIAN Hong-yan, LI Zhi-xin , WANG Hong-jun, ZHU Xin-wei, PAN Xiao-yan, LIU Zhong-ping, LIN Dong-jing Department of Histology and Embryology, J ilin Medical College, J ilin City, J ilin Province 132013, China * Corresponding author, E-mail: lzx-62@163.com This work was supported by the “Twelfth Five-Year P lan” Scientific and Technologic R esearch Project of J ilin Province E ducation Department (2012485, 2012484) [Abstract]　Objective　To investigate the effect of caprine testicular extract on the acrosomal formation of spermatids of lead-treated mice, and find a theoretical and experimental basis for further research on the effects of caprine testicular extract on the reproductive system. Methods The study was carried out on thirty male adult ICR mice, which were randomly assigned into control group, model group and treatment group (10 each). The testis-injury model was reproduced by intragastric administration of lead acetate (30mg/kg, 0.1ml/10g). Animals in treatment group received intragastric administration of lead acetate and intraabdominal injection of 0.5ml caprine testicular extract at the same time for 21days, and the mice in control group received the same amount of distilled water by intragastric administration and 0.