阿尔茨海默病患者PBMCs的TLR4表达改变及意义.pdfVIP

中国现代医药杂志2017年1月第19卷第1期 MMJC，Jan 2017，Vol 19，No.1 · 1 · DOI：10.3969/j.issn.1672-9463.2017.01.001 ·论 著 · 阿尔茨海默病患者PBMCs的TLR4表达改变及意义 张作慧 陈晨 陈浩 崔桂云 花放 【摘要】 目的 分析AD患者外周血单个核细胞TLR4的改变，寻找取材容易、特异性高的生物学标志物。方法 纳 入72例阿尔茨海默病患者作为AD组，60例年龄、性别等具有可比性的健康状况良好的体检者作为对照组。收集相关临 床资料，采集外周血，分离单个核细胞，采用流式细胞术和western blot方法检测TLR4的蛋白表达，采用Real-time PCR 检测其mRNA的表达情况。结果 Real-time PCR结果显示：与对照组相比，AD组TLR4的mRNA表达明显升高（t=3.88， P0.05）。流式细胞术结果显示，AD组TLR4的蛋白表达显著高于对照组，FCM：（31.5±15.1）% vs （10.2±7.5）%，P0.01； western blot结果显示AD组的TLR4蛋白表达显著高于对照组（t=7.301，P0.01）。结论 外周单个核细胞参与阿尔茨海 默病的炎症反应，其TLR4的表达改变有可能作为阿尔茨海默病诊断的生物学标准物，为阿尔茨海默病的诊断提供新思 路。 【关键词】 阿尔茨海默病 外周血单个核细胞 TLR4 MoCA评分 生物学标志物 ThechangeandsignificanceofTLR4 expressiononperipheralbloodmononuclearcellsof patientswithAlzheim⁃ ersdisease ZhangZuohui，ChenChen，ChenHao，etal.TheAffiliatedHospitalofXuzhouMedicalUniversity，Xuzhou 221002 【Abstract】Objective To investigate the changes of TLR4 expression on peripheral blood mononuclear cells （PBMCs）of patients with AD and try to find a convenient and highly specific diagnostic biomarker for AD. Methods 72 AD patients and 60 healthy controls matched for sex and age were recruited. Flow cytometry（FCM）and western blot（WB）were used to test protein expression of TLR4. Real-time PCR was performed to detect the expression of TLR4 mRNA. Results The real-time PCR results showed，compared with control group，mRNA expression of TLR4 was up-regulated in AD patients（t=3.88，P0.05）.The protein level of TLR4 was higher in AD group than in control group [FCM：（31.5±15.1）% vs （10.2±7.5）%，P0.01]；Western blot result showed that the expression of TLR4 protein was significantly higher in AD group than in control group（t=7.301，P0.01）. Conclusion This study indicates that changesofTLR4 expression in PBMCsmay bea diagnostic biomarker forAD. 【Keywords】Alzheimersdisease Peripheral bloodmonon