新型肠球菌万古霉素耐药基因簇vanm基因簇-中国感染与化疗杂志.pdfVIP

436 2009 11 20 9 6 Chin J Inf ect Chemot her , N ov emb er1 2009, V ol1 9, N o1 6 # # ) ) ) vanM 徐晓刚, 吴 湜, 叶信予, 郭 燕, 林东昉, 胡付品, 朱德妹, 王明贵 : 16S r RN A Ef m- HS0 66 1 , ( M L ST ) , P CR , 16S rR N A , M L ST ST 78 , 6 592 bp , 6 , , vanM v anM 1 032 bp, V anM VanA V anBVan D VanF 7 9. 7% 69 . 7% 66 . 0 % 78 . 5 % ) ) ) vanM : : R3 78 . 1 R978 . 1 : A : 10 09-7 70 8( 20 09) 0 6- 0436- 04 vanM gene cluster, a novel vancomyci n resistance gene cluster f ound in Ent erococ- cus f aecium X UX iao-gang , W U Shi, YE X in-yu, G UO Yan, LIN Dong-f ang , H U Fu-p in, ZH UD e-mei, W N G Ming-gui. (Institute of ntibiotics, H uashanH osp ital, Fudan University, Shanghai 200040, China) Abstract: Objective T o clone a no vel v ancomy cin- resistant gene cluster in an clinical isolate of E. f aecium. Methods 16S rRN A sequence analysis, multilocu s sequ ence ty p ing ( M LST ) , antim icro bial su sceptibility testing and conjugation ex per im ent w er e car ried out f or the clinical iso late Ef m- HS0 66 1. By P CR amplif ication , w alking sequ encing , and r estriction frag m ent clo- ning , the vancomycin- r esistant gene and it s flanking sequences w ere deter m ined. T he obtained sequ ence w as comp ared w ith know n vanco mycin resistance gene clu ster s f or sequence identity . Results T he clinical isolate w as identif ied as E. f aecium by 16S r RN A sequencing, w ith M LST typ e ST 78 . T his isolate w as r esistant to vanco mycin and teicoplanin . T he r esistance co uld be tran sf erred by co