肝癌中高通量rna干扰技术及其应用-上海伯豪生物技术有限公司.pdfVIP

1990 , RNA 综述讲座 RNA 1, 2 1,3 徐晓晶 , 殷弘为 1. 生物芯片上海国家工程研究中心功能基因组研究实验室, 上海201203 2. 上海伯豪生物技术有限公司细 蛋白部, 上海201203 3. 美国转化基因组研究所临床转化研究实验室, 亚利桑那州美国AZ 85259 High throughput of RNA interference and its application on hepatocellular carcinoma research X U X iaoj ing 1, 2 , YI N H ongwe i 1, 3 1. F unctional Genom ic R esear ch Div ision, N ational E ng ineer ing Center or B ioch ip o S hang hai , S hang hai 201203, P . R . China 2. Dep ar tment o Cell and P r ote in, Shang hai B io B iotechnology Comp any , Shang hai 201203, P . R . China 3. Clinical Tr anslational R esear ch D iv is io n, T r anslatio nal Genom ics R es ear ch I nstitute , A r iz ona A Z 85259, U S ! :探讨国内外高通量 RNA 干 [ABSTRACT] OBJECTI E: T o revie RNA interference ( RANi) 扰( RNAi) 的技术方法, 及其在寻找新的肝 technology and the application of high throughput RNAi in identifi 癌靶点 治疗方法上的应用: 检索 cation of novel targets and therapeutic treatment s in hepatocellular M edline 及CNKI 期刊全文数据库检索系 carcinoma (HCC) . METHODS: Medline and CNKI databases ere 统, 以∀ RNA 干扰技术肿瘤# 等为关键词, undertaken to identify the relevant articles on high throughput 检索 199901- 201012 的相关文献纳入 RNAi using in HCC research. Publications from 199901 to 2010 标准: 1) 高通量筛选;2) 药物靶点; 3) 肝癌 12 ere searched ith the key ords of ∀ RNA interference, canc 剔除与动物体内实验相关以及技术内容陈 er# . T hen ith the criteria of ∀ high throughput, liver cancer, and 旧的文献根据纳入剔除标准分析39 篇 drug target# , 39 articles ere collected for summarization in this 文献: 高通量的 RNAi 技术是 一种 revie . RESULTS: High throughput RNA interference ( RNAi) , a 良好的基因组水平功能性研究工具, 可以 functional genomics tool, enables