拮抗链霉菌S24抗菌物质的提取及其部分理化性质.PDFVIP

生 物 工 程 学 报 Chin J Biotech 2010, March 25; 26(3): 350–356 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn ©2010 CJB, All rights reserved. 农业生物技术 拮抗链霉菌S24 抗菌物质的提取及其部分理化性质 1 1 1 1 2 1 周启升 ，曹宁宁 ，仇念全 ，张本峰 ，张楠 ，刘训理 1 山东农业大学林学院，泰安 271018 2 山东农业大学生命科学学院，泰安 271018 摘 要: 拮抗链霉菌 S24 对黄曲霉、赭曲霉、黑曲霉等粮食和饲料中常见的曲霉菌具有广谱抗性，本试验通过牛津杯 法测定抗菌物质的效价，研究了大孔吸附树脂对链霉菌 S24 产生的抗菌物质的吸附、解吸性能，筛选了解吸剂，并研 究了抗菌物质的部分理化性质。结果表明，大孔吸附树脂 AB-8 对抗菌物质的吸附及解吸性能最好，其饱和吸附量为 7.0822×104 µg/g ，最佳解吸剂为85 ％丙酮，以85 ％丙酮进行动态解吸，解吸率达93.82 ％。该抗菌物质对热稳定，对紫 外线敏感，对有机溶剂不敏感，对酸敏感，对碱稳定，紫外全波长扫描发现该抗菌物质为多烯大环内酯类抗生素。 关键词: 拮抗链霉菌，抗菌物质，提取，理化性质 Extraction and characterization of antifungal substances produced by antagonistic Streptomyces S24 1 1 1 1 2 1 Qisheng Zhou , Ningning Cao , Nianquan Qiu , Benfeng Zhang , Nan Zhang , and Xunli Liu 1 College of Forestry, Shandong Agricultural University, Taian 271018, China 2 College of Life Science, Shandong Agricultural University, Taian 271018, China Abstract: Streptomyces S24 has broad spectrum resistance to the Aspergillus in food and feed, such as Aspergillus flavus , Aspergillus niger , Asperegillus alutacells and so on. We studied the adsorption and desorption properties of antifungal substance from Streptomyces S24 on macroporous resins, screened the best elution solution and also investigated some physical and chemical characters of antifungal substance by determining the antifugal activity using oxford plate assay system. According to the analysis results, AB-8 resin offered the best adsorption and desorption capacity f