角质酶的研究进展-微生物学报.PDFVIP

生 物 工 程 学 报 Chin J Biotech 2009, December 25; 25(12): 1829-1837 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn © 2009 Institute of Microbiology, CAS CSM, All rights reserved 综 述 1 1 2 1,3 1, 2 李江华 , 刘龙 , 陈晟 , 堵国成 , 陈坚 1 , 214122 2 , 214122 3 , 214122 : 角质酶(EC 3.1.1.74)是一种可以降解角质并产生大量脂肪酸单体的水解酶。角质酶是一种多功能酶, 可水解可 溶性酯、不溶性甘油三酯和各种聚酯, 同时还能催化酸与醇的酯化、脂肪酸盐与醇的转酯化反应, 因此在食品工业、化 工工业等诸多领域都具有广泛的应用。近年来研究发现, 角质酶可实现棉纤维的生物精练和合成纤维的生物改性, 是推 动纺织工业清洁生产的关键酶制剂。 : 角质酶, 角质, 棉纤维的生物精练 Advances in cutinase research 1 1 2 1,3 1,2 Jianghua Li , Long Liu , Sheng Chen , Guocheng Du , and Jian Chen 1 School of Biotechnology, Jiangnan University , Wuxi 214122, China 2 State Key Laboratory of Food Science and Technology, Jiangnan University , Wuxi 214122, China 3 Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China Abstract: Cutinase (EC 3.1.1.74) is a kind of hydrolase capable of catalyzing the cleavage of ester bonds of cutin to release fatty acids. Cutinase displayed hydrolytic activity not only toward cutin but also a variety of soluble synthetic esters, insoluble triglycerides and polyesters. Besides its hydrolytic activity, cutinase also showed synthetic activity and transester activity. Therefore, cutinase was evaluated as a versatile lipolytic enzyme u