慢性超低频复合生理频率电刺激对慢性低压缺氧兔-第三军医大学学报.docVIP

超低频复合生理频率慢性电刺激 对慢性低压缺氧兔颏舌肌肌球蛋白重链表达和肌浆网 Ca2+摄取-释放动力学的影响 刘 熙，刘 刚，张 妮，欧 娜，张 鹏 （第三军医大学西南医院呼吸内科，重庆，400038） [摘要]目的 研究超低频复合生理频率慢性电刺激对慢性低压缺氧兔颏舌肌肌球蛋白重链（myosin heavy chain，MHC）亚型表达和肌浆网（sarcoplasmic reticulum，SR）Ca2+摄取-释放动力学的影响。方法 成年日本大白兔24只，雌雄不限，体重（2.15±0.1）Kg，按随机数字表法分为4组（n=6）：A.正常对照组，B.慢性低压缺氧组，C.2.5Hz电刺激组，D.(2.5+40)Hz电刺激组。B、C、D组兔在构建慢性低压缺氧模型后，给予C、D两组慢性电刺激，刺激频率分别为超低频2.5Hz和超低频复合生理频率(2.5+40)Hz。采用Western印迹法分析4组兔颏舌肌MHC亚型表达变化，采用荧光光度测定法测定SR Ca2+摄取Ca2+摄取Effect of electrical stimulus at a lower physiological frequency on expressions of myosin heavy chain isoforms and kinetics of sarcoplasmic reticulum Ca2+ uptake-release in genioglossus of rabbits with chronic hypoxia Liu Xi，Liu Gang ，Zhang ni，Ou na，Zhang Peng(Department of Respiratory Diseases，Southwest Hospital，Third Military Medical University，Chongqing，40038，China) [Abstract] Objective To study the effect of chronic electrical stimulus at a lower physiological frequency on expressions of myosin heavy chain(MHC) isoforms and kinetics of sarcoplasmic reticulum(SR) Ca2+ uptake-release in genioglossus of rabbits with chronic hypoxia. Methods Twenty-four adult rabbits,weighing 2.15±0.1Kg,were randomized into control group(A),chronic hypoxia group(B),2.5Hz electrical stimulus group(C) and (2.5+40)Hz electrical stimulus group(D).Rabbits in group C and D received electrical stimulus on genioglossus at a frequency of 2.5Hz and (2.5+40)Hz respectively,while those in group B received no electrical stimulus,after they had been fed with free access to food and water in a hypoxia cabin 10 h a day for 4 weeks.Exprseeions of MHC isoforms in genioglossus of rabbits in 4 groups were detected by Western blot,and Fura-2 fluorophotometry was used to assay the kinetics changes of SR Ca2+ uptake-release. Results The expression level of MHCⅡa was significantly higher while that of MHCⅠ was significantly lower in group B than which in group A(P＜0.05).Meanwhile,the genioglossus SR Ca2+ uptake-release velocity in group B was significantly decreased c