干细胞3d 支架的研究进展.pdfVIP

中国生物工程杂志 China Biotechnology 干细胞3D支架的研究进展1 Advances in Stem Cell3D Scaffolds1 徐竹 诸葛启钏 黄李洁2 Xu Zhu，Zhuge Qichuan，Huang Lijie2 （温州医科大学附属第一医院神经外科 温州 325000） （Department of Neurosurgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China） 摘要 干细胞是一类增殖潜力无限，可自我更新的细胞，不同的培养或诱导条件下能够分化成 为不同的细胞类型。目前，随着医学治疗手段以及干细胞研究的不断发展，发现干细胞可运用 于很多疾病的治疗，干细胞临床应用日益广泛,逐渐成为科研工作者研究的热点。然而干细胞移 1 植进入生物体后繁殖效率很低，无法达到需求的量，因此如何对干细胞在生物体外进行大量培 v 养扩增、在生物体内如何更稳定地增殖分化成为迫切需要解决的难题。当前干细胞最主要的培 9 8 养方法仍是2D培养，2D培养无法模拟体内的3D微环境，繁殖效率较低，正是由于2D培养局 6 限性太多，促使国内外学者对3D培养技术和3D支架材料进行深入探索，并取得了大量成果， 0 0 本文便是对3D培养技术在干细胞中的发展与应用进行综述。 . Abstract Stem cells are a class of proliferative potential infinite, self-renewing 7 0 cells that can differentiate into different cell types under different culture or 7 inducedconditions.Atpresent,withthedevelopmentofmedicaltreatmentandstem cell 1 0 research, it is found that stem cells can be used in the treatment of many diseases, 2 and the clinicalapplication of stem cells isbecomingmore andmorepopular.However, : v stem cell transplantation into the living body after the breeding efficiency is very i X low, can not meet the demand, so how to expand stem cells, make them more stable a proliferation and differentiation into an urgent need in vivo iswaiting us to solve. n Currently，the main culture method of the stem cells is still 2D culture. 2D culture i h can not simulate the 3D microenvironment in the body, and the breeding efficiency is c low.Itisbecauseofthelimitationsof2Dculture,whichmakesthedomesticandforeign