几丁聚糖对一次性力竭运动小鼠肝组织自由基代谢影响.doc

几丁聚糖对一次性力竭运动小鼠肝组织自由基代谢影响摘 要：了解几丁聚糖对小鼠一次性力竭运动中自由基代谢的影响。通过建立灌服几丁聚糖小鼠的一次性力竭游泳训练实验模型，测定了小鼠肝组织中超氧化物歧化酶（SOD）的活性和丙二醛（MDA）的水平。实验结果：灌服几丁聚糖后，小鼠游泳运动能力明显提高，（?P<?0.05）；用药组小鼠运动后肝组织SOD活性显著高于运动对照组（P<0.05）；安静组SOD活性显著高于运动组和用药组（?P?<0.05）。灌服几丁聚糖后，小鼠肝组织MDA含量显著低于运动组，（?P?<0.05）；一次性力竭运动后即刻小鼠肝组织MDA含量显著高于安静组（?P?<0 05）。结果表明：一次性力竭运动使机体脂质过氧化反应增加；几丁聚糖具有较强的抗自由基损伤和抗脂质过氧化损伤的作用，服用几丁聚糖能有效地减轻运动所产生的内源性自由基对小鼠肝组织的破坏，增强小鼠的运动能力，延缓疲劳的产生。 关键词：几丁聚糖；力竭运动；自由基；超氧化物歧化酶；丙二醛 中图分类号：G804.7文献标识码：A文章编号：1007-3612(2007)06-0790-03 The Effects of Chitosan on Once Exhaustive Exercise Rats’ Free Radical Metabolism of Liver JIANG Yu?ling?1, YU Yang?2, CUI Jin?ling?2, WANG Bo?2 (1. P.E. College, Shenyang University, Shenyang 110044, Liaoning, China; 2. Shenyang Institute of Physical Education, Shenyang 110032, Liaoning, China) Abstract:To study the effects of chitosan on the delay of fatigue in training rats, by setting up the once exhaustive rats’ model, the activity of liver mitochondria SOD and the quantity of MDA were measured. The results are as follows. After taking chitosan, the physical ability of the rats improved remarkably (?P?<0.05). After training, rats in the chitosan group, the activity of liver mitochondria SOD remained at a higher level than that of the control group (?P?<0.05). The activity of liver mitochondria SOD in resting group have a significant divergence than control groups (?P?<0.05). After the rats took chitosan, the quantity of MDA turned lower than that of the training group(?P?<0.05). After the once exhaustive training, the quantity of MDA appeared superior to that of the resting group (?P?<0.05). The results indicate that lipid peroxidation was increased in or after the once exhaustive training, and chitosan has much stronger influences on the anti?oxidation, anti?free?radical, raising exercise endurance and delaying fatigue in rats. Key words: chitosan; exhaustive exercise; free radical; SOD; MDA 几丁聚糖又称甲壳质、壳聚糖等，俗称甲壳素，是自然界中唯一含游离氨基碱性阳离子的可食性动物纤维，是除蛋白质、脂肪、糖类、维生素和无机盐以外的生命第