大强度运动大鼠运动性蛋白尿形成机制探究.docVIP

大强度运动大鼠运动性蛋白尿形成机制探究摘 要：旨在通过大强度力竭游泳对大鼠肾脏组织代谢的影响来探讨血乳酸、血管紧张素Ⅱ及氧自由基在运动性蛋白尿形成过程中所起的具体作用，以望有益于运动性蛋白尿机制的研究,并为运动强度的监控提供依据。实验发现：1) 大强度运动对肾脏组织代谢产生重大影响，具体表现在该组织内的SOD活性、MDA含量及流经该组织血液中的AngⅡ的活性和BLA浓度都发生了变化。2) 运动后即刻，AngⅡ、HL和自由基可能同时参与了运动性蛋白尿的形成，其中自由基机制、特别是AngⅡ机制可能是引起大分子蛋白尿的主要机制，BLA则可能是导致小分子蛋白尿的主要机制。3) 运动后恢复期，AngⅡ机制、自由基机制可能均参与了该阶段运动性蛋白尿的形成。 关键词：蛋白尿；AngⅡ；白蛋白；β2?微球蛋白；MDA；SOD；BLA 中图分类号：G804.7文献标识码：A文章编号：1007-3612(2007)06-0784-04 Research on the Mechanism of Producing Post?exercise Proteinuria Produced by the Rats Doing Intensive Exercises CHEN Wei?1, TANG Chang?fa?2 (1. Taishan College, Tai’an 271000, Shandong, China; 2. P.E. College, Hunan Normal University, Changsha 410012, Hunan, China) Abstract:After analyzing the influences of heavy exhaustive swimming sport upon rats’ kidney tissue metabolism, the effects of HL, AngⅡ and OFR in the production of post?exercise proteinuria are researched, in order topromote the study on the mechanism of producing post?exercise proteinuria and provide concrete evidences for controlling exercise intensity. The followings are proved by this experiment. 1. Strenuous exercises can affect the normal course of kidney tissue metabolism, which can be seen through the rise of SOD content, MDA content, BLA concentration as well as the activity of AngⅡ in the kidney tissue. 2. Right after exercise, AngⅡ, BLA and OFR may altogether involve in the production of post?exercise proteinuria, especially AngⅡ and OFR may be the main mechanism through which big molecule proteinuria produce, while BLA may be the main mechanism through which small molecule proteinuria is produced. 3. During the recovery period, both AngⅡ and OFR may involve in the production of proteinuria. Key words: post?exercise proteinuria; AngⅡ; albumin; β2?MG; MDA; SOD; BLA 本文旨在通过大强度游泳运动对肾脏组织代谢的影响来探讨血乳酸、AngⅡ及氧自由基在蛋白尿的产生过程中所起的具体作用,以望有益于运动性蛋白尿机制的研究。 1 实验材料与方法 1.1 研究对象及分组 7周龄SD雄性大鼠，体重(214±13)g，具体情况见下表。共30只，为II级实验动物。室温20～25℃，光照时间7：00-19：00，每笼10只饲养，自由饮食、饮水。大鼠及喂养的食料均购于湖南