中药和复方水提物对DPPH·与·OH清除作用.docVIP

中药和复方水提物对DPPH·与·OH清除作用 　　中药及复方水提物对DPPH·和·OH的清除作用 龚永欢，刘 霞，周海霞，李 伟，马 林 （西南科技大学生命科学与工程学院，四川 绵阳 621000） 摘要：采用分光光度法分析测定了具有抗仔猪腹泻作用的12种中药及5个复方的水提物对1，1-二苯基-2-苦基肼自由基（DPPH·）和羟基自由基（·OH）的清除能力，并对其多糖含量进行了测定。结果表明，12种中药及5个复方对DPPH·和·OH均有一定程度的清除作用。浓度在0.2～1.0 mg/mL范围内，秦皮、白头翁、黄连、黄柏以及复方2、4、5对DPPH·清除作用较好，在高浓度时与维生素C对DPPH·的清除作用相当。除复方3外，中药复方在高浓度时对DPPH·的清除率接近或高于其组成中药，表现出协同效应。浓度在5～25 mg/mL范围内，白术、防风、鱼腥草及复方1和复方3对·OH的清除作用较好，复方1和复方3表现出对·OH清除的协同作用。中药及其复方多糖含量与DPPH·和·OH清除作用不存在明显的相关性。 关键词： 抗腹泻中药；抗氧化；1，1-二苯基-2-苦基肼自由基；羟基自由基 中图分类号：R932 文献标识码：A 文章编号：0439-8114（2013）20-4995-05 Scavenging Effect of Aqueous Extracts from Some Anti-diarrhea Chinese Herbs and Compounds on DPPH· and ·OH GONG Yong-huan，LIU Xia，ZHOU Hai-xia，LI Wei，MA Lin （School of Life Science and Engineering， Southwest University of Science and Technology， Mianyang 621000， Sichuan， China） Abstract： Using spectrophotometric and phenol-sulfuric acid method， the scavenging effect on DPPH and hydroxyl radicals， and the polysaccharide contents in the aqueous extracts from 12 Chinese herbs and 5 compounds with anti-diarrhea effects were investigated. The results showed that all 12 Chinese herbs and 5 compounds had scavenging effect on DPPH· and ·OH with different degree. With the concentration of 0.2 to 1.0 mg/mL， the Cortex fraxini， Radix pulsatillae， Rhizoma coptidis and Cortex phellodendri and the compounds 2， 4 and 5 had high scavenging effects on DPPH·. The scavenging effect was close to that of vitamin C at 0.8 mg/mL and 1.0 mg/mL. Except the compounds 3， other compounds had synergistic effect and showed higher scavenging effect on DPPH than that of its component herbs with tested concentration. Under the concentration of 5 mg/mL to 25 mg/mL， the extracts from Rhizoma atractylodis， Radix saposhnikoviae and Houttuynia cordata and compounds 1 and 3 had good scavenging effect on hydroxyl radicals. Similarly， the compounds 1 and 3 showed synergistic effect. There was no significant correlation between the polysaccharide c