Brief Account of Chromatography幻灯片.ppt

Brief Account of Chromatography (P155) New words Gas chromatography chromatography: method of separating chemicals by porous medium and analyzing compounds. Gas chromatography Read the paragraph 1~2, and find out: Two major types of GC Two types of column stationary phases Compound be analyzed by GC What is capacity factor? Gas chromatography Gas chromatography apparatus Gas chromatography detector Gas chromatography Column bleeding HPLC Advantage over gas chromatography apparatus columns Detectors spectrophotometric method 分光光度法 Detectors Deuterium lamp 氘灯 Xenon 氙 Diode array detectors 光电二级管阵列检测器 Differential refractometer 差示折光计 Fluorometric detectors 荧光检测器 HPLC-grade solvents and reagents Methanol acetonitrile * * color chromato- chromo- chromosome, chromatin skin dermato- dermo- dermatitis dermatology dermal dermoid a carrier gas source an injection port column detector recording device Temperature-controlled Temperature-controlled Flame-ionization detectors NP detectors-a selective detector thermionic source: alkali-metal salt sensitive to organic nitrogen and phosphorus Capillary column Split injection Splitless mode Pumping systems injectors columns detectors Data collection devices Schematic of an HPLC instrument normal phase chromatography reverse-phase chromatography Ion-exchange chromatography Size-exclusion chromatography spectro-: spectrum photo-: light metric-: -metry (n) , a kind of method Fluorescence- fluorometry iodine –iodimetry bromide- bromimetry *