北五味子叶总黄酮的提取及生物活性-河南农业科学.pdfVIP

72 2013, Vol.34, No.20 食品科学 ※工艺技术 北五味子叶总黄酮的提取及生物活性 许瑞波，万蓓蓓，高颖颖，章 蕊，周洪英 (淮海工学院化工学院，江苏 连云港  222005) 摘  要：以北五味子叶为原料，用单因素试验考察提取温度、乙醇体积分数、料液比、提取时间及提取次数对北 五味子叶总黄酮(FSL)提取率的影响。在单因素试验的基础上，通过正交试验设计优化FSL提取的工艺条件，确定 最佳提取工艺参数。结果表明：最佳工艺条件为提取温度85℃、乙醇体积分数60% 、料液比1:45(g/mL)、提取时间 3.5h、提取2次。在此条件下，FSL提取率为9.03% 。用聚酰胺树脂初步提纯，经红外光谱表征，证明其具备黄酮类 化合物的基本光谱特征。此外，利用琼脂扩散抑菌法研究了FSL对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的抑 制作用；并利用邻苯三酚法和DPPH法研究了FSL 的抗氧化活性。结果表明，PSL具有良好的抑菌作用，对超氧阴离 子自由基和DPPH 自由基具有一定的清除能力。 关键词：北五味子叶；黄酮类化合物；提取；抑菌作用；抗氧化活性 Extraction and Bioactivities of Total Flavonoids from Schisandra chinensis (Turcz.) Baill Leaves XU Rui-bo ，WAN Bei-bei ，GAO Ying-ying，ZHANG Rui ，ZHOU Hong-ying (School of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, China) Abstract ：The effect of extraction temperature, ethanol concentration, solid/solvent ratio, extraction time and number of extraction cycles on the yield of total flavonoids from Schisandra chinensis (Turcz.) Baill leaves (FSL) was studied by single factor designs. Based on the results obtained, an orthogonal array design was proposed to optimize the extraction process. The optimal extraction process was obtained by two cycles of extraction with 60% ethanol at a solid/solvent ratio of 1:45 (g/mL) for 3.5 h at 85 ℃, resulting in an FSL yield of 9.03%. Primary purification of FSL was performed using polyamide resin, and infrared spectroscopic studies demonstrated that the purified products possessed spectral characteristics of flavonoids. The purified FSL had strong inhibitory effects against Staphylococcus aureus, Escherichia coli and Bacillus subtilis as determined by agar diffusion method, and showed free radical scavenging activity against superoxide anion and DPPH radicals.