北五味子种子和果肉中的活性成分比较.pdfVIP

※分析检测 食品科学 2012, Vol. 33, No. 16 175 李立顺，时维静* ，马迅兵 (安徽科技学院食品药品学院，安徽 凤阳 233100) 目的：比较北五味子种子和果肉中活性成分的差异。方法：采用薄层色谱法比较北五味子种子、果肉 和果实中木脂素成分含量；采用苯酚 - 硫酸法测定种子、果肉和果实及果肉不同组分糖的含量；薄层色谱法分析 三氟乙酸水解后，果肉不同组分单糖组成。结果：北五味子木脂素类主要在种子中；北五味子在果肉和种子中的 总糖含量分别为 169.34、5.366mg/g；果肉中各组分糖含量分别为醇溶组分 117.06mg/g、醇沉组分75.67mg/g、多糖 组分 12.37mg/g。三氟乙酸水解后薄层分析，醇溶组分主要由葡萄糖组成，多糖组分主要由葡萄糖、半乳糖组成。 结论：北五味子种子和果肉中活性成分存在明显差异。 北五味子；种子；果肉；木脂素；多糖 Comparative Analysis of Bioactive Component in Seeds and Flesh of Schisandrae chinensis Fructus by Thin Layer Chromatography LI Li-shun ，SHI Wei-jing*，MA Xun-bing (School of Food and Drug, Anhui Science and Technology University, Fengyang 233100, China) Abstract ：Objective: To comparatively analyze bioactive components in seeds and flesh of Schisandrae chinensis Fructus . Methods: The contents of lignan in seeds, flesh and whole fruit of Schisandrae chinensis Fructus were determined by high performance thin layer chromatography (HPTLC). Sulfuric acid-phenol method was used to quantify sugar. After trifluoroacetic acid hydrolysis, the monosaccharide composition of the flesh was analyzed by HPTLC. Results: Lignans in Schisandrae chinensis Fructus were mainly present in the seeds, and total sugar content was 169.34 mg/g in the flesh and 5.366 mg/g in the seeds. The alcohol- soluble, alcohol-insoluble and polysaccharide components were 117.06, 75.67 mg/g and 12.37 mg/g in the fresh, respectively. The alcohol-soluble fraction was mainly composed of glucose. The polysaccharide fraction was mainly composed of glucose and galactose. Conclusion: Bioactive components reveal a significant difference between seeds and flesh of Schisandrae chinensis Fructus. Key words