拟南芥草酸不敏感突变体的筛选与分析-微生物学报.pdfVIP

生 物 工 程 学 报 Chin J Biotech 2008, February 25; 24(2): 203-208 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ © 2008 Institute of Microbiology, CAS CSM, All rights reserved 研究报告 1, 2 2 1 1 2 1, 2 陈晓婷 , 陈芳芳, 陈立群, 郑麟 , 鲁国东 , 王宗华 1 , 350002 2 , 350002 : 草酸是多种真菌的致病因子。在含 1.2 mmol/L 草酸和 10 μmol/L 雌二醇的 MS 缺钙培养基上, 从大约含 6000 个独立株系的拟南芥化学诱导突变体库中筛选草酸不敏感的突变体。初筛获得的可能的草酸不敏感突变体单株收种后, 进一步复筛获得 5 株较抗草酸的突变体 D33 、D74 、D154 、D282 和 D630 。对它们的 TAIL-PCR 的第三步产物回收、测 序、比对的结果表明：D33 的 T-DNA 插入位点位于 At2g39720 (Zinc finger ) and At2g39730 (Rubisco activase) 之间, D74 、 D154 、D282 和 D630 都插在 At5g10450 (14-3-3 protein GF14 lambda) 的第一个内含子上。突变体后继的遗传分析与分 子分析正在进行中。 : 拟南芥, 突变体筛选, 草酸不敏感, TAIL-PCR Isolation and Analysis of Oxalic Acid Insensitive Mutant of Arabidopsis thaliana 1, 2 2 1 1 2 1, 2 Xiaoting Chen , Fangfang Chen , Liqun Chen , Lin Zheng , Guodong Lu , and Zonghua Wang 1 College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2 Key Laboratory of Bio-pesticide and Chemistry Biology, Ministry of Education , Fuzhou 350002, China Abstract: Oxalic acid (OA) is inhibitory to many fungal plant pathogens. To further characterize the molecular mechanism of OA involved in fungal pathogenesis, OA insensitive mutants were screened from a chemical inducible Arabidopsis mutant library (about 6000 lines) using MS medium (calcium free) containing 1.2 mmol/L OA a