深山含笑和乐昌含笑叶片挥发油的成分分析及性能测定-生物质化学工程.pdfVIP

第42卷第4期 生摇 物摇 质摇 化摇 学摇 工摇 程 Vol.42 No.4 2008年7月 Biomass Chemical Engineering July2008 深山含笑和乐昌含笑叶片挥发油的 成分分析及性能测定 1 1* 2 2 邱金芬 ,何开跃 ,李晓储 ,黄利斌 (1.南京林业大学森林资源与环境学院, 江苏 南京 210037;2.江苏省林业科学院, 江苏 南京 211153) 摘摇 要:采用超临界二氧化碳萃取法提取深山含笑和乐昌含笑叶片挥发油,应用GC-MS技术测定挥发油的成分并对其 DPPH 自由基清除能力和还原能力等抗氧化指标进行测定。 结果表明:深山含笑萜类为35.35%,酯类为15.12%,醇类 为9.09%,其余37.02% 为萜烯类含氧衍生物。 乐昌含笑萜类为28.49%,酯类为 11.12%,醇类为 1.94 %,其余 54.55%为萜烯类含氧衍生物。 两种含笑叶片挥发油质量浓度为50mg/ L时DPPH 自由基清除能力达到饱和,且挥发油 质量浓度越大还原能力越强。 通过与常用抗氧化剂进行比较,发现两种含笑叶片挥发油的抗氧化能力均强于植酸PA、 没食子酸丙酯PG和抗坏血酸Vc。 关键词:超临界二氧化碳;深山含笑;乐昌含笑;挥发油;性能 中图分类号:TQ91摇 摇 摇 摇 摇 摇 摇 文献标识码:A摇 摇 摇 摇 摇 摇 摇 文章编号:1673-5854(2008)04-0017-06 Compositions and Properties of Volatile Oils from Leaves of Michelia maudiae Dunn and Michelia chapensis Dandy 1 1 2 2 QIUJin鄄fen ,HE Kai鄄yue ,LI Xiao鄄chu ,HUANG Li鄄bin (1. College of Forest Resources and Environment,Nanjing Forestry University,Nanjing210037,China; 2. Jiangsu Academy of Forest,Nanjing211153,China) Abstract:The volatile oils from Michelia maudiae Dunn and Michelia chapensis Dandy leaves were extracted by supercritical carbon dioxide. Their compositionswere detected by GC鄄MS ,meanwhile antioxidation activities such as scavengingrateon DP鄄 PHfree radical and reducing power were determined. Results show that the constitutes of M. maudiae were terpene 35.35%, ester 15.12% and alcohol 9.09%. The rest 37.02% were terpene oxydirivatives. the constitutes ofM. chapensis wereter鄄 pene 28.49%,ester 11.12% and alcohol 1.94 % . The rest 54.55% areterpene oxydirivatives. When the concentrations of the two volatile oils were 50mg/ L,the sca