红叶李与安哥诺李及其杂交子代的rapd分析-中国农学通报.pdfVIP

中国农学通报 2011,27(8): 194-198 Chinese Agricultural Science Bulletin 红叶李与安哥诺李及其杂交子代的RAPD分析 李 单，孟庆瑞，沙翠芸，刘 冰，李盼华，李彦慧 （河北农业大学园林与旅游学院，河北保定071000） 摘 要：利用随机扩增多态性DNA 分子标记（RAPD ）技术对红叶李与安哥诺李及其杂交子代中的10株 植株进行遗传多样性分析。从100 个随机引物中筛选出15 个引物，然后对所有子代进行分析。利用 PopGene3.2 软件分析所得图谱的多态位点数、多态位点百分率、Shannon 信息指数(I)、Neis 基因多样性 指数(H)、遗传距离和遗传一致度。用DPSv2.0 软件依据所得到的遗传距离，按照非加权平均距离法 （UPGMA ）构建树状图。结果表明：15 个随机引物共扩增出多态条带89 个，总的多态位点百分率为 93.54% ，平均Shannon 信息指数为0.4298 ，平均Neis 基因多样性指数为0.3574 ，说明杂交后代具有较丰 富的遗传多样性。从树状图可以看出杂交子代的遗传变异较丰富，其中1号植株与母本亲缘关系最近， 最有可能稳定遗传母本的红叶性状。 关键词：红叶李；安哥诺李；杂交子代；RAPD ；遗传多样性 中图分类号：S722.3+4 文献标志码：A 论文编号：2010-3228 TheRAPDAnalysisofP.salicina×P.atropurupureaand Prunussalicinacv. ‘angenuo’anditsHybrids Li Dan, Meng Qingrui, Sha Cuiyun, Liu Bing, Li Panhua, Li Yanhui (CollegeofLandscapeArchitectureandTourism,AgricultureUniversityofHebei, Baoding Hebei 071000) Abstract:The random amplified polymorphic DNA (RAPD) markers technique was used to analyze the genetic diversity among P. salicina ×P. Atropurupurea and Prunus salicina cv. ‘angenuo ’and 10 hybrids. 15 primers were screened from 100 random primers, and they were amplified in all hybrids. The polymorphic bands, the percentage of polymorphic loci, Shannon information index (I), Nei ’s genetic diversity index (H), genetic distance and genetic identity were analyzed by using PopGene3.2 software. The dendrogram based on genetic distance was constructed by using DPSv2.0 software, in accordance with the un-weighted pai-group method using an arithmetic average (UPGMA). The results showed that 15 primers amplified 89 polymorphic bands, the total percentage of polymorphic loci was 93.54%, the average Shannon information i