电刺激初级听皮层对水杨酸耳鸣模型大鼠下丘外侧核神经元放电的影响.PDFVIP

生理学报 Acta Physiologica Sinica, April 25, 2009, 61 (2): 121-126 121 研究论文 电刺激初级听皮层对水杨酸耳鸣模型大鼠下丘外侧核神经元 放电的影响 宋海燕，童钟，王毅敏，钱善军，郭瑞新，施建蓉* 上海中医药大学科技实验中心，上海 201203 摘 要：本研究通过制作急性水杨酸(salicylate acid, SA)大鼠耳鸣模型，观察电刺激初级听皮层(primary auditory cortex, AI)对 下丘外侧核(external nucleus of inferior colliculus, ICx)放电活动的影响，探讨听觉的下行调控对耳鸣相关放电活动的作用。 采用立体定位技术及细胞外记录方法，记录电刺激左侧AI 后同侧ICx 放电活动变化，用平均自发放电率和放电间隔直方图 为观察指标。电刺激AI 前、后持续记录急性SA 模型大鼠同一ICx 神经元自发放电。结果显示，电刺激AI 对较高放电率 的ICx 神经元起抑制作用，对较低放电率的ICx 神经元起易化作用。从ICx 神经元平均自发放电率和短间隔放电比例来分 析，电刺激AI 后的4 h 观察时间内，对正常组与急性SA 模型组大鼠ICx 神经元放电均起抑制作用，而对急性SA 模型组 大鼠的抑制作用比对正常大鼠的抑制作用持久。上述结果提示，电刺激AI 通过下行通路调控可在一段时间内抑制SA 引起 的与耳鸣相关的ICx 放电活动。 关键词：水杨酸；初级听皮层；下丘外侧核；耳鸣；自发放电；离皮层调制 中图分类号：R339.16 Effect of electrical stimulation of the primary auditory cortex on the spontaneous activities of the external nucleus of the inferior colliculus in a rat model of tinnitus induced by salicylate acid SONG Hai-Yan, TONG Zhong, WANG Yi-Min, QIAN Shan-Jun, GUO Rui-Xin, SHI Jian-Rong* Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China Abstract: This study aims to investigate corticofugal modulation on spontaneous activities of the external nucleus of the inferior colliculus (ICx) in a salicylate acid (SA) induced tinnitus rat model by the stimulation of the primary auditory cortex (AI). Extracellular recording techniques and stereotaxic method were used. The spontaneous activities of a single unit were recorded from the left ICx after electrical stimulation was given to the left AI of the rats duplicated as acute SA models. The average rate of spontaneous discharge and the interspike interval histogram of spontaneous activities were used as indices. The single unit spontaneous discharges of the same unit of ICx before and after AI stimulation were observed. There was an inhibitory effect of AI stimulation on the activities of the high discha