蟾酥注射液对免疫抑制小鼠免疫功能的调节作用.PDFVIP

中国农学通报 2011,27(14):45-49 Chinese Agricultural Science Bulletin 蟾酥注射液对免疫抑制小鼠免疫功能的调节作用 郭维霄，仲伟婷，李文华，杨小丰，杨振国 （吉林大学畜牧兽医学院，长春130062） 摘 要：为研究蟾酥注射液对免疫抑制小鼠免疫功能的调节作用，采用免疫药理学方法制造免疫低下的 动物模型，测定脾脏重量并计算脾脏指数；ELISA 方法检测IgG ，IgG ，IgG 抗体水平；MTT 法检测脾淋 1 2b 巴细胞增殖反应；ELISA 方法检测细胞因子（IFN-γ、IL-4 、IL- 10）水平。测定结果表明，蟾酥注射液能够 提高免疫低下小鼠脾脏指数，提高抗体水平；在ConA 或者LPS 刺激下提高脾细胞增殖率，促进淋巴细 胞分泌细胞因子，说明蟾酥注射液在一定剂量下能够缓解氟苯尼考引起的免疫抑制。 关键词：氟苯尼考；蟾酥注射液；OVA ；免疫抑制；细胞因子 中图分类号：S8 文献标志码：A 论文编号：2010-3655 RegulativeEffectsofBufonisParenteralonImmunosuppressioninMice GuoWeixiao,ZhongWeiting,LiWenhua,YangXiaofeng,YangZhenguo (College of Animal Science and Veterinary Medicine, Jilin University,Changchun130062) Abstract: In the present study, regulative effects of bufonis parenteral sloution on-induced immunosuppressioninmicewereevaluated.Animalmodelof florfenicol-inducedimmunosuppressionwas made.Slpeenswereweightedandcalculatedweightindex.OVA-specificIgG,IgG,IgG weremeasuredby 1 2b ELISA,splenocyteswereharvestedfordetermininglymphocyteproliferationandspleenlymphocytesecrete cytokinesweremeasuredbyELISAmethod.Theresultsshowedthatbufonisparenteralsolutioncouldincrease theweightandthelevelofantibody.Bufonisparenteralsolution,treatedwithpolyclonalT,Bcellmitogen(Con AorLPS)inacertaindoserange,enhancedtheproliferationsignificantly.Bufonisparenteralsolutionalso induces cytokines secretion in vivo. The results indicated that bufonis parenteral solution possessed the regulativeeffectsonflorfenicol-inducedimmunosuppressioninmice.