双波长法测定常用淀粉原料中直链淀粉支链淀粉及总-广东农业科学.PDFVIP

广东农业科学 2013 年第18 期 97 双波长法测定常用淀粉原料 直链淀粉、 支链淀粉及总淀粉 量 刘襄河 郑丽璇 郑丽勉 欧成成 叶超霞 王安利 渊华南师范大学生命科学学院/广东省水产健康安全养殖重点实验室/ 广东省高等学校生态与环境科学重点实验室 广东 广州 510631冤 摘要 建立了双波长法测定常用淀粉原料中直链淀粉尧支链淀粉和总淀粉含量的方法遥 按照双波长测定的等吸收点作图法 确定2种淀粉的测定波长和参比波长 直链淀粉的测定波长分别为630尧480 nm 支链淀粉的测定波长分别为550尧735 nm遥 结果表 明 直链淀粉浓度在0~36 滋g/mL尧支链淀粉浓度在40~100 滋g/mL范围内线性关系良好 符合比耳定律 具有较高的精确度和准确 度遥常用淀粉原料的总淀粉含量在66.51%~83.65% 其中绿豆淀粉和豌豆淀粉的直链淀粉含量比明显高于其他淀粉原料曰糯米粉中 的支链淀粉含量最高 其直链淀粉含量过低 导致吸光值太小而检测不出遥 关键词 淀粉原料曰双波长分光光度法曰直链淀粉曰支链淀粉 中图分类号 TS237 文献标识码 A 文章编号 1004-874X渊2013 冤18-0097-04 Determination of amylose and amylopectin in the commonly used starch materials by dual-wavelength spectrophotometry LIU Xiang-he, ZHENG Li-xuan, ZHENG Li-mian, OU Cheng-cheng, YE Chao-xia, WANG An-li (College of Life Science , South China Normal Unive rsity / G uangdong Prov inc ial Key Laboratory for Healthy and Safe A quaculture / Key Laboratory of Ecology and Env ironment Scie nce in G uangdong Higher Education, Guangzhou 510631, China) Abstract: A new method for the determination of amylose and amylopectin in the commonly used raw materials was established based on dual-wavelength spectrophotometry. According to the principle of dual-wavelength spectrophotometry method, the contents of amylose and amylopectin in the common raw materials of starch were determined at the determination wavelength of 630 nm and 550 nm with the reference wavelength of 480 nm and 735 nm, respectively. The content of amylose and amylopectin could be figured out from two regressive equations. Beer law was obeye