新孢子虫病rELISA抗体检测试剂盒地应用.pdfVIP

新疆农业科学 2011, 48( 5) : 942- 947 Xinjiang Agricultural Sci nc s 新孢子虫病rELISA 抗体检测试剂盒的应用 陈亮, 刘启生, 杨 帆, 曹雯丽, 巴音查汗 ( 新疆农业大学动物医学学院, 乌鲁木齐 830052) :rELISA rELISA , rELISA , , , , 1 329 rELISA , , 90% 1 329 157 , 11. 8% , : ; rELISA; ; + : S852. 72 3, S188 : A : 100 1- 4330( 20 11) 05- 0942- 06 Application of an Indirect rELISA Kit for the Detection of Antibody against Neosp ora Caninum CHEN Liang, LIU Qi- sh ng, YANG Fan, CAO W n- li, Bayin Chahan ( College of Veterinary Medicine, Xinj iang Agricultural University , Urumqi 830052, China) Abstract:ObjectiveTo d v lop a prov n and stabl indir ct rELISA kit for d t ction of antibody against N eosp ora caninum MethodAn indir ct rELISA kit for th d t ction of antibody against Neosp ora caninum was d v lop d bas d on th optimal r action conditions which w r s l ct d pr viously. Th d v lop d kit was valuat d for s nsitivity, r t ntion p riod, sp cificity and r producibility, and compar d with two kinds of comm rcial ELISA kits. Blood sampl s w r coll ct d from 1 329 animals in s v ral susp ct d ar as of Xinjiang Uyghur Autonomous R gion and t st d th m for antibodi s against N eosp ora caninum by th kit. ResultAn indir ct rELISA kit for d t ction of antibody against Neop ora caninum was d v lop d. Th kit has long r t ntion p riod, high sp cificity, s nsitivity, and r producibility. Th agr m nts b tw n th d v lop d kit and two kinds of comm rcial ELISA kits w r mor than 90%. On hundr d and fifty s v n s rum sampl s w r positiv among 1 329 sampl s, and th s ropr val nc was 11. 8%. onclusionTh kit can b a substitut for comm rcial ELISA kits to t st antibodi s against Neosp ora caninum , and a t chniqu for to pr v ntion of th N osporosis is provid d. Key words: N