基于srap标记的刺葡萄亲缘关系分析-湖南农业大学学报欢迎您！.pdfVIP

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基于srap标记的刺葡萄亲缘关系分析-湖南农业大学学报欢迎您！.pdf

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基于srap标记的刺葡萄亲缘关系分析-湖南农业大学学报欢迎您！

38 6 () Vol.38 No.6 2012 12 Journal of Hunan Agricultural University (Natural Sciences) Dec 2012 DOI:10.3724/SP.J.1238.2012.00607 基于SRAP标记的刺葡萄亲缘关系分析 1,2 1,2 1,2* 1,2 1,2 1,2 (1. 410128 2. 410128) 摘要SRAP 21 84 SRAP 7 61 34 55.7% NTSYS 21 0.800.990.92 3 UPGMA 6 1 9 (2003 3 ) 关键词SRAP 中图分类号S321 文献标志码A 文章编号1007–1032(2012)06–0607–05 Relationship analysis for Vitis davidii Foëx with SRAP molecular markers LIU Kun-yu1,2, XU Feng1,2, SHI Xue-hui1,2*, ZHONG Xiao-hong1,2, YANG Guo-shun1,2, NI Jian-jun1,2 (1.College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China; 2.Hunan Provincial Engineering Research Center for Grapes, Changsha 410128, China) Abstract: Sequence-related amplified polymorphism (SRAP) molecular markers were used to detect the genetic diversity and relationship of 21 accessions of Vitis davidii Foëx. From 84 pairs of primers, 7 pairs were selected out. Sixty-one clear bands were amplified using these 7 pairs of primers, of which 34 were stable morphological bands, accounting for 55.7% of total bands. Revealed by NTSYS software, the genetic similarities of 21 accessions ranged from 0.80 to 0.99. Cluster analysis showed that 21 accessions were classified into 3 cluster groups with genetic similarity of 0.92. UPGMA cluster analysis showed that there are 6 types of Vitis davidii Foёx in Hunan, and we successfully deduced the suspected parents (which were lost during 3-time relocations) of Nongda No.1 to No.9 which come from different regions of Vitis davidii Foёx seeds by colchicine treatment. Key words: Vitis davidii Foёx; relationship; sequence related amplified polymorphism (Vitis davidii Foëx) [5] [6–8]