应用jar细胞株建立scidbeige小鼠绒毛膜癌移植瘤病理模型.pdfVIP

·1406 · doi 10.3969/j.issn. 1673-4254.2015.10.08 J South Med Univ, 2015, 35(10): 1406-1410 基础研究 应用应用JARJAR 细胞株建细胞株建立立SCIDSCID beigebeige 小鼠绒毛膜癌移植瘤病理模型小鼠绒毛膜癌移植瘤病理模型 田 泉，郑 伟，孙 戎，薛 艳，季 伟，安瑞芳 西安交通大学医学院第一附属医院妇产科，陕西 西安 710061 摘要：目的建立稳定有效的绒毛膜癌SCID beige 小鼠移植瘤模型，观察模型的病程特点和生物学行为。方法 将3~5 周龄 SCID beige雌性小鼠随机分为实验组和对照组；实验组12只小鼠分为A（皮下移植瘤模型）/B （肺转移瘤模型）两组，分别皮下注 6/只，应用形态学、放射性免疫分析法测定β-HCG、小动物活体成像系统（IVIS）和组织学HE 染色 射或尾静脉注射JAR 细胞5×10 方法明确JAR 细胞皮下移植瘤及肺转移瘤形成等情况。结果 实验组接种28 d后，A 组小鼠皮下形成质硬肿瘤结节，HE 染色符 合绒毛膜癌细胞形态学表现；B 组小鼠应用IVIS 检测显示体内出现单个或多个肿瘤实体包块，HE 染色表明肺组织均有不同程 度的瘤细胞浸润。接种第14天，实验组β-HCG 水平显著高于对照组（P0.05）；其中B 组β-HCG 水平明显高于A 组（P0.05）。 结论 SCID beige小鼠皮下/尾静脉注射JAR 细胞可成功构建人绒毛膜癌移植瘤病理模型，该模型能模拟临床绒毛膜癌上皮性 实体瘤特性及肺转移特点，是研究人类绒毛膜癌发病机理及实验治疗的良好模型。 关键词：绒毛膜癌；SCID beige小鼠；JAR 细胞；移植瘤模型；活体成像系统 Establishment of a SCID beige mouse model bearing transplanted human choriocarcinoma using JAR cell line TIAN Quan, ZHENG Wei, SUN Rong, XUE Yan, JI Wei, AN Ruifang Department of Gynecology and Obstetrics, First Affiliated Hospital, Xi ’an Jiaotong University College of Medicine, Xian 710061, China Abstract: Objective To establish a mouse model bearing human choriocarcinoma xenograft in severe combined immuno-deficient (SCID) beige mice and investigate the disease course and biological behaviors of the tumors．Methods Human choriocarcinoma JAR cells were injected in female SCID beige mice (3-5 weeks old) either subcutaneously (group A, n= 6) or via the tail vein (group B, n=6). Morphological studies, radioactive immunoassay, in vivo tumor imaging and histopathological examinations were performed to confirm JAR cell engraftment at the subcutaneous injection site and in the lungs of the mice. Results On day 28 after tumor cell inoculation, the mice in group A showed palpable subcutaneous nodules, and HE staining reveal