离子色谱法测定奶粉中的葡萄糖、蔗糖和乳糖.PDFVIP

176 2012, Vol. 33, No. 08 食品科学 ※分析检测 离子色谱法测定奶粉中的葡萄糖、蔗糖和乳糖 熊建飞，周光明* ，许 丽，张 磊 (发光与实时分析教育部重点实验室，西南大学化学化工学院，重庆 4007 15) 摘 要：建立高效阴离子交换色谱- 脉冲安培检测法测定奶粉中葡萄糖、蔗糖和乳糖的方法。以METROSEP CARB 1( 150mm ×4 .0mm) 阴离子交换柱为分离柱，脉冲安培检测，37mmol/L NaOH 溶液为淋洗液，以柠檬酸作为蛋白 质沉淀剂。葡萄糖、蔗糖和乳糖的线性范围分别为1～4 0 、1～50 、1～50mg/L ，相关系数分别0 .99 66 、0 .99 6 8 、 0.9985 ，检出限分别为0 .0 14 、0 .09 1、0.083mg/L ，精密度为1.10% ～4 .96% ，回收率为90 .13%～104 .83% 。该方 法分析时间短，前处理简单，适用于快速测定奶粉中的葡萄糖、蔗糖和乳糖。 关键词：高效阴离子交换色谱；脉冲安培检测；奶粉；葡萄糖；蔗糖；乳糖；柠檬酸 Determination of Glucose, Sucrose and Lactose in Milk Powder by Ion Chromatography 　 XIONG Jian-fei ，ZHOU Gu ang-ming * ，XU Li ，ZHANG Lei (Key Laboratory on Luminescence and Real-time Analysis (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 4007 15, China) Abstract ：A high performance anion exchange chromatographic-pulse amperometric detection method was established to determine glucose, sucrose and lactose in milk powder . The separation column u sed was METROSEP CARB 1 ( 150 mm × 4.0 mm) anion exchange column, and the mobile phase was 37 mmol/L NaOH. Citric acid was used to precipitate proteins from milk. The linear ranges of glucose, sucrose and lactose were 1－40, 1－50 mg/L and 1－50 mg/L with correlation coefficients of 0.9966, 0.9968 and 0.9985, respectively , and the detection limits were 0.0 14, 0.09 1 mg/L and 0.083 mg/L . The precision relative standard deviations of precision for 5 replicate determinations were in the range of 1.10% －4.96%. The recovery rates for glucose, sucrose and lactose in 4 different commercial samples were 90.13% －104.83%. The method was suitable for rapid d