酸解玉米芯制备木糖及其提纯工艺的研究 study on production and purfication of xylose from corncob by acid hydrolysis.pdfVIP
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酸解玉米芯制备木糖及其提纯工艺的研究 study on production and purfication of xylose from corncob by acid hydrolysis
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酸解玉米芯制备木糖及其提纯工艺的研究
郭仁杰。孙婉。赵灵希,熊海容‘
(中南民族大学生命科学学院,湖北武汉430074)
摘要:探讨玉米芯的酸解工艺及木糖的纯化过程。玉米芯经1%NaOH60℃预处理24h后,最优酸解条件为硫酸浓
ho溶液经洛l生炭脱色和树脂去离子,由酿酒酵母As2.541去除木糖母液中的葡
度1.5%,料zgflM:13(g/mL).反应时问3
萄糖。纯化后的糖液经薄层层析(TLc)鉴定其单糖种类,3-5-群i基水杨酸法(DNS)测定还原糖总量,高效液相法
(HPLc)做糖液的定量分析,最后将糖液浓缩醇沉,制备木糖结晶率为1.5%,纯度为_90%。
关键词:玉米芯;木糖;酸解;纯化
onProductionandPurficationof fromCorncobAcid
Study Xylose by Hydrolysis
GUO Wan,ZHAO
Ren-jie,SUN Ling-xi,XIONGHai-rong
ofLife Central for
Science,South Nationalities,Wuhan430074,Hubei,China)
(College University
Abstract:Thisresearchwasto theacid ofcorncobto andits
explore hydrolysisprocess producexylose
Was 60℃for24
thecorncob 1%NaOHat h.the acid
pretreated optimal
purificationprocedure.After by
solutionis usesulfuricacidconcentrationat1.5%for3hreactionwiththefeed/waterratioof
to 1:13(g/mL).
Afterdeeolorationactivatedcarbonanddesalination in solutionWasthen
resin,the
by by glucosehydrolysis
removed cerevisiaestainAs2.541 resultofthin
bySaccharomyces consumption.Thelayerchromatography
showedthe inthe solutionWas total
(TLC)assaymonosaecharide sugar
type purified mainlyxylose.The
reductioncontentwasmonitoredand and
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