CG冲激的小鼠骨髓树突状细胞的生物学活性变化.PDFVIP

《现代免疫学))2005年第25卷第1期 ·37· H HSP7OBCG冲激的小鼠骨髓树突状细胞的生物学活性变化 崔向丽，施广霞，郭连英，沈 洁，钱振超 f大连医科大学肿瘤生物治疗研究所，大连 116027) 摘要：利用H HSP70 、HT^一HSP70w_G体外冲激小鼠骨髓树突状细胞，观察树突状细胞的抗原捕获能力及抗原提呈能力的 变化。用流式细胞仪检测不同冲激组树突状细胞对FITC．Dextran的摄取率；用 MTF法检测不同冲激组树突状细胞对HCa—F 的增殖抑制作用、对脾不黏附细胞的刺激增殖活性及活化后的脾不黏附细胞对HCa．F的杀伤活性；分别．【}j ELISA法和一氧 化氮硝酸还原酶法检测树突状细胞培养上清中II，l2和NO的浓度。结果显示，冲激后的树突状细胞对FITC—Dextran的摄取 率降低．对HCa—F的增殖抑制率、脾不黏附细胞的刺激增殖指数、活化后的脾不黏附细胞对HCa—F的杀伤率及树突状细胞 培养上清中IL-12和NO的浓度均较对照组树突状细胞增高(P0，05)。结果表明HT^．HSP70 ：及J 独H 、HSP70~ 均能促进小 鼠骨髓树突状细胞的成熟，成熟后的树突状细胞抗原捕获能力下降，而抗原提呈能力增强 关键词：树突状细胞；热休克蛋白：卡介苗；抗原提呈；抗瘤免疫 中图分类号：R392．12 文献标识码：A 文章编号：100l一2478(2005)叭．0037．05 The changes in biological activities of the mouse bone marrow—derived dendritic cells pulsed with H HSP70BOG CUI Xiang—li，SHI Guang—xia，GUO Lian—ying，SHEN Jie，QIAN Zhen—chap(Institut ‘Cancer Biotherapy， Dalian Medical，Dalian 116027，China) Abstract：The mouse bone marrow—derived dendritic cells(DC) were pulsed with H ，HSP70I or HT，r HSP70瞄 ，and their antigen capturing and antigen presenting capacities were evaluated．The FITC—labeled dextran taking—over capacity of distinct dendritic cells pulsed was determined by flow cytometry，and the inhibition rate of the HCa—F proliferation by DC．the proliferation rate of non—adherent splenocytes stimulated with distinct dentritic cells pulsed as well as the cytotoxiclty activities of the activated non—adherent splenocytes were all evaluated by means of MTr assay．The content of IL一1 2 and concentration of N0 in the cuhural supernatants of DC were measured by ELISA and the NO reagent box respectively．The results showed that the phagocytosis rate of the FITC labeled dextran of pulsed DC．the inhibition rate HCa—F proliferation by pulsed DC， the stimulation index of the non—adherent splenocytes by pulsed DC． the cytotoxicity activity of the activate