蛇足石杉内生真菌清除DPPH·和抑制乙酰胆碱酯酶活性探究.docVIP

蛇足石杉内生真菌清除DPPH·和抑制乙酰胆碱酯酶活性探究 　　摘要：为了从蛇足石杉[Huperzia serrata （Thunb.）Trev.]中分离出能产生清除DPPHDPPH自由基、抑制乙酰胆碱酯酶活性的能力，形态学和ITS-rDNA序列分析法鉴定获得的部分内生真菌。结果表明，共分离得到94株内生真菌，有31株内生真菌发酵产物显示出乙酰胆碱酯酶抑制活性，其中18株具有显著的抑制活性， 关键词：蛇足石杉[Huperzia serrata （Thunb.）Trev.]；DPPH 中图分类号：S567 文献标识码：A 文章编号：0439-8114（2014）04-0888-06 Effects of Endophytic Fungi Isolated from Huperzia serrata on Scavening DPPH and AchE Inhibitory DONG Li-hui1，FAN San-wei1，LING Qing-zhi1，QIAN Hai-feng1，WEI Zhao-jun2 （1.Zhejiang Pharmaceutical College， Ningbo 315100，Zhejiang，China； 2.School of Biotechnology and Food Engineering， Hefei Universuity of Technology，Hefei 230009，China） Abstract： The endophytic fungi isolated from Huperzia serrate by plate were fermented and identified by morphological characters and ITS-rDNA sequence. The effects of the extracts from fermented broth and mycelium on DPPH scavenging ratio and inhibition ratio for AchE were investigated. The results showed that 94 endophytic fungis were isolated from Huperzia serrate 31 strains of them exhibited inhibition activity to AChE， while 18 strains isolated from stem（9）， leave（8） and root（1） had significan inhibition rate ranged from 50.3% to 82.78%. The fermentation of 5 strains such as S22， S29， L44， S79 and S94 had both activity of AchE-inhibitory and antioxidant. Through ITS-rDNA sequence analysis the five strains were identified as Arthrinium sp.， Podospora sp， Trichoderma sp.， Colletotrichum sp. and Colletotrichum sp.， respectively. It is indicated that there are endophytic fungi with antioxidant and AchE inhibitory activity in Huperzia serrate. Key words： Huperzia serrate； DPPH?； AChE； endophytic fungi 蛇足石杉[Huperzia serrata（Thunb.）Trev.]属于石杉科（Huperiaceae）石杉属（Huperzia Bernh.）的蕨类植物，近年的研究[1-5]表明，蛇足石杉的主要药效成分――石杉碱甲（Huperzine A）是一种强效的乙酰胆碱酯酶（Actylcholinesterase，AchE）抑制剂，用于治疗重症肌无力，能改善老年性记忆功能减退，与其他同类产品相比，副作用小[6]，是目前临床上用于治疗老年性痴呆症（Alzheimers disease，AD）的最有效的药物。研究表明，石杉碱甲能够保护神经元细胞对抗β-淀粉样蛋白产生的毒性，并提高超氧化物歧化酶（SOD）的产生[7，8]。据研究报道，石杉碱甲能通过刺激超氧化物歧化酶