右江流域壮族人群酒精依赖程度与ADH和ALDH基因多态性的关联性研究.docVIP

右江流域壮族人群酒精依赖程度与ADH和ALDH基因多态性的关联性研究 　　【摘要】目的研究右江流域?炎迦巳阂掖纪亚饷福?ADH）和乙醛脱氢酶（ALDH）基因多态性的分布，为筛选壮族酒精高易感性人群提供参考。 　　方法通过问卷进行流行病学调查，明确右江流域壮族人群饮酒状况；采用聚合酶链式反应限制性片段长度多态性（PCRRFLP）技术，在分子生物学层面，检测对照组（100例）和酗酒组（90例）ADH和ALDH基因型并明确其在样本中的分布。 　　结果问卷调查结果显示，严重依赖酒精的占17.8%，月均饮酒量3000 ml以上的占16.7%；对照组ADH2基因型中异常纯合型（异纯型）32例，占32.0%，酗酒组ADH2基因型中异纯型6例，占6.7%，与对照组比较差异有统计学意义（P0.001）。对照组ALDH2基因型中纯合型20例，占20.0%，酗酒组ALDH2基因型中纯合型62例，占68.9%，与对照组比较差异有统计学意义（P0.001）。 　　结论低异纯型ADH2基因频数和高纯合型ALDH2基因频数在壮族酗酒人群中具有特征性，可供筛选壮族酒精高易感性人群参考。 　　[HJ1*3][HJ]【关键词】壮族；乙醇脱氢酶；乙醛脱氢酶；基因多态性 　　中图分类号：R345.41；R394.2文献标识码：ADOI：10.3969/j.issn2017.01.007 　　[HJ1*3][HJ] 　　A study on relationship between alcohol dependence and gene polymorphisms of ADH and ALDH in Zhuang population in Youjiang Basin 　　[HJ1*3][HJ] 　　LI Keming，TANG Hanqing▲，ZHAO Yufeng，ZHAO Qiuhua，ZHENG Jianzhou，ZHU Xiaoying，DOU Xibin 　　（Key Laboratory of Ethnic Minorities of Guangxi Universities，Youjiang Medical University for Nationalities，Baise 533000，China） 　　[HJ1*3][HJ] 　　【Abstract】ObjectiveTo explore the distribution of gene polymorphisms of alcohol dehydrogenase（ADH） and acetaldehyde dehydrogenase（ALDH） in Zhuang nationality in Youjiang Basin and provide reference to filter high alcohol sensitive Zhuang nationality people. 　　Methods Epidemiological investigation was conducted to determine drinking status of Zhuang people in Youjiang Basin.Polymerase chain reaction restriction fragment length polymorphism（PCRRFLP） technique was used to detect ADH and ALDH genotypes of control group（100 cases） and excessive drinking group（90 cases） in molecular biology，and distributions of them in the sample were defined. 　　ResultsQuestionnaire results showed that patients who heavily depended on alcohol accounted for 17.8%，and monthly average alcohol consumption over 3000 ml accounted for more than 16.7%.In the control group，there were 32 cases of ADH2 abnormal homozygotic genetype，accounting for 32.0%，while there were 6 cases of ADH2 abnormal homozygotic genetype in t