光谱法研究痂囊腔菌素a与血红蛋白的相互作用-南京师范大学学报.pdfVIP

33 2 () V ol. 33 No. 2 2010 6 JOU RNAL OF NAN JING NORM AL UN IV ERSITY ( N a ural Sc ience Edi ion) Jun, 2010 光谱法研究痂囊腔菌素 A 与血红蛋白的相互作用 1 1 1 1 1 2 1 马 菲 , 周 林 , 王 薇 , 冯玉英, 周家宏, 王兴和, 沈 健 ( 1. , , , 2 10046 ) ( 2. , 2 10046 ) [ ] A ( EA ) ( Hb) . , EA H b H b, , EA H b , EA H b . H b , EA H b . EA Hb. [ ] A, , , , [ ] O 6573 [ ] A [] 2010) Spectroscopic Studies on Interaction of E lsinochrome A W ith Hemoglobin 1 1 1 1 1 2 1 M a Fe i , Z hou L in , W ang W e i, Feng Y uy ing , Z hou J iahong , W ang X inhe , Shen J ian ( 1. K ey Lab of B iofun c ion M a erials of Jiangsu Provin ce, A naly s is and T es ing C en er, N anjing N orm al U n iversi y, N anjing 2 10046, Ch ina) ( 2. S chool of Phy sics and T echnology, N anjing N orm al U n ivers iy, N anjing 2 10046, C hina) Abstract: The in e rac ion be w een E lsinochrome A ( EA ) and hem og lob in ( H b) w as inves ig a ed us ing UVv is spec ros copy and fluorescence spec roscopy. T he resu l s sugges ed ha he in e rac ion m odesw ere obv iou sly differen in he dark and he illum ina ed cond i ion. In he dark, EA in e rac ed w ih he surface am ino ac id of H b on ly, bu w i h he illum ina ion, bo h am idic ac id and he in e rio r hem achrom e o f Hb in e rac ed w ih EA. A cco rding o he va lues of he quenching cons an and he hermodynam ics param e ers, he quench ing m echan ism w as dynam ic quenching procedure and he in er ac ion be w een EA and H b w as m a inly hydrophobic in na ure. The chang es in he m icroc ircum s ance o f