夏枯草黄酮对去卵巢大鼠骨质疏松的抑制作用.docx

夏枯草黄酮对去卵巢大鼠骨质疏松的抑制作用刘华1，钟业俊2，吴丹3（1.江西科技师范大学生命科学学院，江西南昌330047）（2.南昌大学食品科学与技术国家重点实验室，江西南昌330013）（3.江西省药品审评中心，江西南昌330047）摘要：本研究通过干预去卵巢大鼠骨质疏松动物模型，探讨夏枯草黄酮对骨质疏松大鼠骨量、生物力学性能及骨代谢的影响。经夏枯草黄酮处理后，取血清检测血矿物质、护骨素（OPG）、碱性磷酸酶量（ALP）的含量变化，取各组股骨进行骨密度测定和骨组织形态检测，并用荧光定量PCR的方法检测Ⅰ型胶原、整合素β1与粘着斑激酶的mRNA表达。结果显示，夏枯草黄酮组ALP（3.03±0.22IU/L）、破骨细胞数（0.23±0.051/mm2）、骨吸收周长百分数（14.94±5.12%）低于模型组（P0.05），而OPG量（186.34±44.21pg/mL）、骨密度、骨小梁相对体积（26.11±5.32%）和厚度（587.16±165.01?m）与模型组相比则有所增加（P0.05）；表明夏枯草黄酮能够提升去卵巢大鼠的成骨细胞的功能，减缓骨吸收和骨代谢，促进骨形成，降低骨小梁损失，抑制骨量减少与骨强度降低，从而提升去卵巢大鼠的骨密度，最终对骨质疏松症起到了抑制作用。关键词：夏枯草黄酮；骨质疏松；骨组织形态测量；骨密度；护骨素；碱性磷酸酶；mRNA表达文章篇号：1673-9078(2014)8-6-11InhibitoryEffectofPrunellavulgarisL.FlavonoidsonOsteoporosisinOvariectomizedRatsLIUHua1,ZHONGYe-jun2,WUDan3(1.SchoolofLifeScience,JiangxiScienceTechnologyNormalUniversity,Nanchang330047,China)(2.TheNationalKeyLaboratoryofFoodScience,NanchangUniversity,Nanchang330013,China)(3.JiangXiProvincialCenterForDrugEvaluation,Nanchang330047,China)Abstract:Inthisstudy,theeffectofflavonoidsfromPrunellavulgarisL.onbonemass,biomechanicalproperties,andbonemetabolismofovariectomized,osteoporoticratmodelwasinvestigated.AftertreatmentwithflavonoidsextractedfromP.vulgarisL.,ratserumsampleswerecollectedtoexaminechangesinthelevelsofbloodminerals,osteoprotegerin(OPG),andalkalinephosphatase(ALP).Thefemurbonewasalsocollectedfromratsineachgrouptomeasurebonemineraldensity(BMD)andbonetissuemorphology.AfluorescentquantitativepolymerasechainreactionapproachwasusedtodeterminetheexpressionlevelsofcollagentypeI,integrinβ1,andfocaladhesionkinasemRNA.TheresultsshowedthatthelevelofALP(3.03±0.22IU/L),numberofosteoclasts(0.23±0.051/mm2),andboneresorptionperimeterpercentage(14.94%±5.12%)werelowerintheflavonoidsgroupthaninthecontrolgroup(p0.05).Incontrast,thelevelsofOPG(186.34±44.21pg/mL),BMD,andtherelativevolume(26.11%±5.32%)andthickness(587.16±165.01μm)oftrabecularbonewerehigher(p0.05)intheflavonoidsgroupthaninthecontrolgroup.TheresultsindicatedthatPrunellavulgarisL.flavonoidsenhancedosteoblastfunction