响应面法优化微波法提取甘青青兰总黄酮工艺.docx

响应面法优化微波法提取甘青青兰总黄酮工艺杨娟霞，董 伟，韩林磊，夏从芳，高云涛，叶艳青*( 云南民族大学化学与生物技术学院，民族药资源化学国家民委－ 教育部重点实验室，云南昆明 650500)摘 要:目的: 研究甘青青兰总黄酮的最佳提取工艺。方法: 以甘青青兰为原料，比较振荡提取法、超声提取法以及微波提取法，得出微波法具有提取优势。在单因素的基础上，采用响应面法优化微波法提取总黄酮，分别选定微波时间、 微波温度、乙醇浓度和液料比四个水平进行响应面实验，通过回归分析得到优化组合条件。结果: 甘青青兰总黄酮微 波提取最佳工艺条件为: 微波时间 7min，微波温度 59℃ ，乙醇浓度 27% ，液料比 25∶1mL / g，此条件下总黄酮实际得率为29.68% ，与模型预测得率 29.65% 的相对误差为 0.1% 。结论: 此优化方法可行，为甘青青兰总黄酮的提取提供了参考数据。关键词:甘青青兰，总黄酮，响应面法，微波提取Optimization of microwave extraction of flavones inHerba Dracocephali Tangutici using the response surface methodYANG Juan－ xia，DONG Wei，HAN Lin－ lei，XIA Cong－ fang，GAO Yun－ tao，YE Yan－ qing*( Key Laboratory of Chemistry in Ethnic Medicinal Ｒesources，State Ethnic Affairs Commission ＆ Ministry of Education，School of Chemistry and Biotechnology，Yunnan University of Nationalities，Kunming 650500，China)Abstract: Objective: To investigate the optimal extraction conditions of flavones from Herba Dracocephali Tangutici.Methods: Herba Dracocephali Tangutici as the raw material. Flavones were extracted by different ways such asOscillatio，ultrasonic and microwave，and the extraction effects were compared. The results indicated thatmicrowave extraction had the advantage of higher efficiency.Four extraction parameters including microwave time，microwave temperature，ethanol concentration and liquid / material ratio were optimized using response surfacemethodology based on single factor experiments for achieving maximum flavones extraction rate. Ｒesults: Ｒesults showed that these optimal extraction conditions were as follows: microwave time of 7min，microwave temperature of59℃，ethanol concentration of 27% ，and liquid / material ratio of 25 ∶ 1mL / g. Under those conditions the actual yield rate of flavones was 29.68% ，and the relative error between the predicted yield 29.65% was 0.1%. Conclusion: The results demonstrated that this method was feasible，and provided reference data for the extraction of flavones from Herba Dracocephali Tangutici.Key words: Herba Dracoceph