基于核磁共振技术的定量代谢组学研究quantitative-药学学报.pdfVIP

药学学报 Acta Pharmaceutica Sinica 20 14, 49 (7): 949 955 ·949 · ·综述· 于核磁共振技术的定量代谢组学研究 江春迎, 王映红* ( 中国医学科学院、北京协和医学院 物研究所, 天然药物活性物质与功能国家重点实验室, 北京 100050) 摘要: 核磁共振技术 (NMR) 既可用于混合体系的定性分析, 又可以用于其定量分析。在过去的几十年里, 随着分析技术以及各种实验技术的迅速发展, 基于核磁共振的定量分析方法已广泛应用于生物样本的分析。核 磁共振定量分析技术应用于代谢组学, 并成为定量代谢组学 (quantitative metabolomics) 研究中的重要手段。本 文将论述这种新分析方法相比于传统方法的优势及不足之处, 同时论述其研究过程中需考虑的重要因素以及其 在代谢组学研究中的应用。 关键词: 核磁共振; 代谢; 代谢组学 中图分类号: R9 17 文献标识码: A 文章编号: 0513-4870 (20 14) 07-0949-07 Quantitative metabolomics based on NMR * JIANG Chun-ying, WANG Ying-hong (State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Ac ademy of Medical Sciences and Peking Union Medical College, Beijing 100050, China ) Abstract : Nuclear magnetic resonance (NMR) spectroscopy can be used to both identify and quantify chemicals from complex mixtures. Over the last several decades, significant technical and experimental advances have made quantitative nuclear magnetic resonance (qNMR) a valuable analytical tool for quantitative measurements of a wide variety of samples. This particular approach is now being exploited to characterize the metabolomes of many different biological samples and is called quantitative metabolomics or targeted metabolic profiling. In this review, some of the strengths, limitations of NMR-based quantitative metabolomics will be discussed as well as the practical consid