废次烟叶中绿原酸的提取与分离 Extraction and Separation of Chlorogenic Acid from Waste and Sub-standard Tobacco.pdfVIP

烟草科技／TobaccoScience＆Technology烟草化学／TobaccoChemistry 废次烟叶中绿原酸的提取与分离 古君平。魏万之’ 湖南大学化学生物传感与计量学国家重点实验室，长沙市岳麓区麓山南路2号410082 关键词：废次烟叶；绿原酸；HPD-400大孔树脂 摘要：考察了提取溶剂种类、乙醇浓度、料液比、提取时间、提取溶剂pH值，以及HPD-400大孔树脂吸附 与脱附条件对废次烟叶中绿原酸提取与分离的影响。结果表明：①超声辅助提取的适宜条件为：提取溶 剂30％(pH4)乙醇，料液比1：10，提取时间45min；②烟叶绿原酸提取液的层析条件为：层析柱：HPD一 mL 400大孔树脂柱；上柱液浓度4．0 mL；流速1．42mL／min；洗脱液191．7 mg／mL；上柱液体积：42．5 30％乙醇水溶液。采用此条件，10．1 g废次烟叶样品可提取0．335g固形物，其绿原酸含量为31．9％， 绿原酸提取率为1．71％。层析回收率72．3％。 中图分类号：Ts411．1文献标识码：B文章编号：1002—0861(2010)02—0043—05 Extractionand of AcidfromWasteandSub-standardTobacco SeparationChlorogenic GUJUN-PINGandWEIWAN．ZHI State of and 41 KeyLaboratoryChemo／BiosensingChemometrics，HunanUniversity，Changsha0082，China and resin sub—standard Keywords：Waste tobacco；Chlorogenicacid；HPD-400macroporousadsorptive effectsof as of Abstract：The concentration，ratio experimentalconditions，suchextractingsolvent，alcohol materialto valueof conditionof solvent，and adsorption—desorptionby liquid，extractingtime，pH extracting from HPD-400 theextractionand of acid wasteandsub·standard macroporousresin，on separationchlorogenic ofextractionassisted tobaccowere resultsshowed conditions investigated．The that：1)theappropriate by ultrasonicwere solvent ofmaterialto 1：1 time45 extracting30％(pH4)ethanol，ratioliquor0，extraction